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Larvicidal activity of the aqueous extract of the clove (Eugenia caryophyllata) against Aedes aegypti (Diptera, Culicidae) under laboratory conditions.
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Keywords

Eugenia caryophyllata
aqueous extract
larvicide
bioassay
Aedes aegypt

How to Cite

ES, M., FM, C., IB, R., & WP, T. (2011). Larvicidal activity of the aqueous extract of the clove (Eugenia caryophyllata) against Aedes aegypti (Diptera, Culicidae) under laboratory conditions. Journal of Research in Biology, 1(7), 518-523. Retrieved from https://ojs.jresearchbiology.com/index.php/jrb/article/view/136

Abstract

In the search for new agents capable to control the dengue vector, Aedes aegypti, bioassays were performed to assess the larvicidal activity of aqueous extract of clove (Eugenia caryophyllata) under laboratory conditions. Mortality data obtained at 24, 48, and 72 h intervals were subjected to probit analysis to determine the lethal concentrations of the extract. Larval mortality ranged from 43 to 100% among the concentrations were sampled (25 x 103, 50 x 103, 100 x 103, 150 x 103, and 200 x 103 ppm). LC50 and LC90 values were: 29.7 x 103 and 63.5 x 103 ppm for 24 h; 232 x 103 and 39.5 x 103 for 48 h; and 22.0 x 103 and 33.7 x 103 ppm for 72 h, respectively. The LC50 value for the 24 h reading differed from the others by presenting a high chi-square value; therefore, the LC50 value for 48 h was used as reference. These results suggested that the clove extract presents bioactive substances capable of killing mosquito larvae.

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References

Aguilera L, Navarro A, Tacoronte J, Leyva M, Marquetti MC. 2003. Efecto letal de Myrtaceas cubanas sobre Aedes aegypti (Diptera: Culicidae). Rev Cubana Med Trop., 55:100-104.

Brasil-Funasa. 2001. Fundação Nacional de Saúde. Dengue: instruções para pessoal de combate ao vetor: manual de normas técnicas. 3ª. Ed., Ministério da Saúde, Brasília.

Carvalho AFU, Melo VMM, Craveiro AA, Machado MIL, Bantim MB, Rabelo EF. 2003. Larvicidal Activity of the Essential Oil from Lippia sidoides Cham. Against Aedes aegypti Linn. Mem Inst Oswaldo Cruz, 98:569-571.

Cavalcanti ESSE, Morais SM, Lima MAA, Santana EWP. 2004. Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Mem Inst Oswaldo Cruz, 99:541-544.

Consoli RAGB, Lourenço-de-Oliveira R. 1994. Principais mosquitos de importância sanitária no Brasil. Fiocruz, Rio de Janeiro.

Costa JGM, Rodrigues FFC, Angélico MR. 2005. Estudo químico-biológico dos óleos essenciais de Hyptis martiusii, Lippia sidoides e Syzigium aromaticum frente ás larvas do Aedes aegypti. Rev Bras Farmacogn., 15:304-309.

Dulmage HT, Yousten AA, Singer S, Lacey LA. 1990. Guidelines for productions of Bacillus thuringiensis H -14 and Bacillus sphaericus. UNDP/World Bank/WHO, Steering Committee to Biological Control of Vectors, Geneva.

Finney DJ. 1981. Probit analysis. 3rd. Ed., S. Chand & Company Ltd., Ran Nagar, New Delhi.

Forattini OP. 2002. Culicidologia Médica. Identificação, biologia, epidemiologia. Ed., Edusp, São Paulo. 2:1a.

Furtado RF, Lima MGA, Andrade-Neto M, Bezerra JNS, Silva MGV. 2005. Atividade Larvicida de Óleos Essenciais Contra Aedes aegypti L. (Diptera: Culicidae). Neotrop Entomol., 34:843-847.

Gonçalves MEC, Oliveira JV, Barros R, Torres JB. 2001. Efeito de extratos vegetais sobre estágios imaturos e fêmeas adultas de Mononychellus tanajoa (Bondar) (Acari: Tetranychidae). Neotrop Entomol., 30:305-309.

Gubler DJ. 2004. The changing epidemiology of yellow fever and dengue. Comp Immunol Microbiol Infect Dis., 27:319-330.

Gusmão DS, Páscoa V, Mathias L, Vieira IJC, Braz-Filho R, Lemos FJA. 2002. Derris (Lonchocarpus) urucu (Leguminosae) extract modifies the peritrophic matrix structure of Aedes aegypti (Diptera: Culicidae). Mem Inst Oswaldo Cruz., 97:371-375.

Han MK, Kim SI, Ahn YJ. 2006. Insecticidal and antifedant activities of medicinal plant extracts against Attagenus unicolor japonicus (Coleoptera: Dermestidae). J Stored Prod Res., 2:15-22.

Kelecom A, Rocha MA, Majdalani EC, Gonzalez MS, Mello CB. 2002. Novas atividades biológicas em antigos metabólitos: ácido oleanólico e eugenol de Eugenia caryophyllata. Rev Bras Farmacogn., 12:70-71.

Kettle DS. 1992. Medical and Veterinary Entomology. C.A.B. International, Wallingford, Oxon, UK.

LeOra Software. 1987. POLO-PC: a user’s guide to Probit or Log analysis. Berkeley, CA.

Lima JB, Da-Cunha PM, Da-Silva RC, Galardo AK, Soares SS, Braga IA, Ramos RP, Valle D. 2003. Resistance of Aedes aegypti to organophosphates in several municipalities in the States of Rio de Janeiro and Espirito Santo, Brazil. Am J Trop Med Hyg., 68:329-33.

Luna JS, Santos AF, Lima MRF, Omena MC, Mendonça FAC, Bieber LW, Sant’Ana AEG. 2004. A study of the larvicidal and molluscicidal activities of some medicinal plants from northeast Brazil. J Ethnopharmacol., 97:199-206.

Marcoris ML, Andrighetti MT, Takuku L, Glasser CM, Garbeloto VC, Bracco JE. 2003. Resistance of Aedes aegypti from the state of São Paulo, Brazil, to organophosphates insecticides. Mem Inst Oswaldo Cruz., 98:703-708.

Mendonça FAC, Silva KFS, Santos KK, RibeiroJúnior KAL, Sant’Ana AEG. 2005. Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti. Fitoterapia 76:629- 636.

Pérez-Pacheco R, Hernández CR, Lara-Reyna J, Belmont RM, Valverde GR. 2004. Toxicidad de aceites, esencias y extractos vegetales em larvas de mosquito Culex quinquefasciatus Say (Diptera: Culicidae). Acta Zool Mex., 20:144-152.

Pinheiro VCS, Tadei WP. 2002a. Frequency, diversity, and productivity study on the Aedes aegypti most preferred containers in the city of Manaus, Amazonas, Brazil. Rev Inst Med Trop S Paulo 44:245-250.

Pinheiro VCS, Tadei WP. 2002b. Evaluation of the residual effect of temephos on Aedes aegypti (Diptera: Culicidae) larvae in artificial containers in Manaus, Amazonas State, Brazil. Cad Saúde Pública 18:1529-1536.

Pohlit AM, Quignard ELJ, Nunomura SM, Tadei WP, Hidalgo AF, Pinto ACS, Santos EVM, Morais SKR, Saraiva RCG, Ming LC, Alecrim AM, Ferraz AB, Pedroso ACS, Diniz EV, Finney EK, Gomes EO, Dias HB, Souza KS, Oliveira LCP, Don LC, Queiroz MMA, Henrique MC, Santos M, Lacerda-Júnior OS, Pinto PS, Silva SG, Graça YR. 2004. Screening of plants found in the State of Amazonas, Brazil for larvicidal activity against Aedes aegypti larvae. Acta Amazo., 34:97-105.

Silva HHG, Silva IG, Santos RMG, RodriguesFilho E, Elias CN. 2004. Atividade larvicida de taninos isolados de Magonia pubescens St. Hil. (Sapindaceae) sobre Aedes aegypti (Diptera, Culicidae). Rev Soc Bras Med Trop., 37:396-399.

Silva HHG, Silva IG, Souza SS, Guimarães VP, Elias CN, Pimenta JF. 2001. Estudo comparative de eficiência das técnicas de ultra baixo volume (UBV) e termonebulização (FOG) no controle de Aedes aegypti. Inf Epidem SUS 10:45-46.

Simas NK, Lima EC, Conceição SR, Kuster RM, Oliveira-Filho AM. 2004. Produtos naturais para o controle da transmissão da dengue – atividade larvicida de Myroxylon balsamum (óleo vermelho) e de terpenóides e fenilpropanóides. Quim Nova 27:46-49.

World Health Organization – WHO. 1981. Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO/VBC/81.807

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