Abstract
The study investigated the optimum conditions of temperature, pH, inoculum size and time of incubation on bacterial protease production. Protease producing bacterial species were isolated from abattoir soil and identified as Pseudomonas aeruginosa and Staphylococcus aureus. The optimum conditions observed for protease production was 37 oC at pH 9, with 2 % inoculum in the medium for 24 h of incubation. Pseudomonas aeruginosa had higher protease activities at optimum temperature and pH than Staphylococcus aureus while S. aureus had higher protease activities at optimum incubation time of 24 h and inoculum concentration of 2 %. The study gave evidence that these bacterial isolates could be potentially applied in biotechnological processes
References
Adinarayana K, Jyothi B and Ellaiah P. 2005. Production of Alkaline Protease with Immobilized cells of Bacillus subtilis PE-11 in Various Matrices by Entrapment Techniques. AAps. J. Pharm. Sci. Technol., 6:391-397.
Amara AA, Salem SR and Shabeb MSA. 2009. The possibility to use bacterial protease and lipase as biodetergent. Global J. Biotechnol. Biochem., 4(2):104-114.
Anson ML. 1938. The Estimation of Pepsin, Trypsin, Papain and Cathepsin with Hemoglobin. J. Gen. Physiol., 22:79-89.
Dalev PG. 1994. Utilization of Waste Feathers from Poultry Slaughter for Production of a Protein Concentrate. J. Biores. Technol., 48:265-267.
Elibol M, Antouio R and Moreira S. 2005. Optimizing some factors affecting alkaline protease production in a marine bacterium Terclinobacter turbirae under solid substrate fermentation. Process Biochem., 40:1951-1956.
Folin O and Ciocalteau VJ. 1929. On Tyrosine and Tryptophan Determinations in Proteins. J. Biol. Chem., 73:62-650.
Gupta R, Beg QK and Lorenz P. 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol., 59(1):15-32.
Gupta A, Joseph B, Moni A and Thomas G. 2007. Biosynthesis and properties of an extracellular thermostable serine alkaline protease from Virgibacillus panthenticus. J. Microbiol. Biotechnol., 36:417-426.
Holt JG, Krieg NR, Sneath PHA, Staley JT, 1994. Williams T. Bergey’s Manual of Determinative Bacteriology. 9th ed. Williams and Wilkins, Baltimore, USA. 1-50.
Hossain MT, Akter S. Rahman MS. Manchur MA and Anwar MN. 1999. Isolation of protease producing fungi and their protease activity. The Chittagong Univ. J. Sci., 23:101-6.
Kalaiarasi K and Sunitha PU. 2009. Optimization of alkaline protease production from Psuedomonas fluorescens isolated from meat waste contaminated soil. Afri. J. Biotechnol., 8(24):7035-7041.
Kammoun R, Naili B and Bejar S. 2008. Application of a statistical design to the optimization of parameters and culture medium for a-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding byproduct). Biores. Technol., 99:5602-5609.
Kumar A, Sachdev A, Balasubramanyam SD and Saxena AK. 2002.Optimization of Conditions for Production of Neutral and Alkaline Protease from Species of Bacillus and Pseudomonas. J. Microbiol., 42:233-236.
Marzan LW, Manchur MA, Hossain MT and Anwar MN. 2004. Protease production by a strain Aspergillus funiculosus. Bangladesh J. Life Sci., 16:103-7.
Mohen FN and Dilee D. 2005. Potential Application of Protease Isolated from Pseudomonas aeruginosa PD 100. J. Biotechnol., 8:197-203.
Ravel H and Banerjee SE. 2003. Effect of Enzyme and Chemical Pretreatments on the Properties of Silk. J. Text. Assoc., 64:65-69.
Sally NJ. 2000. Purification and characterization of a novel protease from Burkholderia strain 2.2 N. M.Sc Thesis, Microbiology Department, Virginia Polytechnic Institute and State University, USA. 34-60.
Sathees Kumar R, Probhu D, Shankar T, Sankaralingam S and Anandapandian KTK. 2011. Optimization of alkalophilic protease by Pseudomonas aeruginosa isolated from the gut of Penaus monodon. World J. Fish and Marine Sci., 3(5):371-375.
Sharma P, Goel R and Capalash N. 2007. Bacterial laccases. World J. Microbiol. Biotechnol., 23:823-832.
Shumi W, Hossain MT and Anwar MN. 2004. Isolation and purification of Aspergillus funiculosus G. Smith and its enzyme protease. Pakistan J. Biol.Sci., 7: 312-7.
Srinubabu G, Lokeswari N and Jayaraju K. 2007. Screening of nutritional parameters for the production of protease from Aspergillus oryzae. EJournal of chem., 4(2):208-215.
Thangam BE and Rajkumar GS. 2002. Purification and characterization of alkaline protease from Alcaligenes faecalis. Biotechnol. Appl. Biochem., 35:149-54.
Tambekar DH and Tambekar SD. 2011. Partial characterization and optimization of protease production from newly isolated Cohnella thermotolerans from Lonar Lake. Journal of research in Biology 4:292-298.
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