Journal of Research in Biology Biology Journal Journal of Biology Biology research journal biomedical journal
Radio-protective and anti-clastogenic effects of Barleria lupulina Lindl. extract against γ (gamma)-ray (1.2 Gy) induced mitotic chromosomal aberrations of laboratory mice Mus musculus and it’s effect on fish tumor induced after γ- irradiation
PDF
HTML

Keywords

Barleria lupulina Lindl
γ (gamma)-ray
chromosomal aberrations
mice bone marrow cells
radiation protection
anti-clastogenic
anti-tumor
anti-cancer

How to Cite

Sur, P. K., & Das, P. K. (2012). Radio-protective and anti-clastogenic effects of Barleria lupulina Lindl. extract against γ (gamma)-ray (1.2 Gy) induced mitotic chromosomal aberrations of laboratory mice Mus musculus and it’s effect on fish tumor induced after γ- irradiation. Journal of Research in Biology, 2(5), 439-447. Retrieved from https://ojs.jresearchbiology.com/index.php/jrb/article/view/235

Abstract

Cancer and tumor formation are a common problem to mankind all over the globe. Cancer is a life threatening disease. In this study, we have investigated for radio-protection by using Barleria lupulina Lindl. plant extract (BLPE) on mice, one hour pre and one hour post treated with γ -ray (1.2 Gy dose). Adult healthy mice were subjected to whole body γ -irradiation (1.2 Gy). Different types of mitotic chromosomal aberrations were studied. Out of the two sets, maximum protection was obtained with BLPE post treated animals (72.22%). Analysis of the data revealed that BLPE can give significant protection to mice against chromosomal damage induced by γ-ray. Moreover, thin layer chromatography and phytochemical analysis revealed the presence of steroid, terpenoid, glycosides, flavonoid, tannins and carbohydrate, in the BLPE. BLPE was also found to reduce γ-ray induced tumor in fish (Oreochromis mossambicus). Thus we report for the first time potent anti-clastogenic and anti tumor activity of BLPE in mice and fish, respectively.

PDF
HTML

References

Donna GA, Colin Co, Frank McC, Joe WG. 2003. Chromosome aberrations in solid tumors. Nat Genet 34:369-376.

Doss A, Parivuguna V, Vijayasanthi M and Sruthi Surendran. 2011. Antibacterial evaluation and phytochemical analysis of certain medicinal plants, Western Ghats, Coimbatore. Journal of Research in Biology. 1:24-29.

Jagetia GC, Venkatesha VA, Reddy TK. 2003. Naringin, a citrus flavonone, protects against radiation-induced chromosome damage in mouse bone marrow. Mutagen 18(4):337-343.

Kanchanapoom T, Kasai R, Yamasaki K. 2001. Iridoid glucosides from Barleria lupulina. Phytochem 58:337-341.

Kim HK, Choi IJ, Kim CG, Kim HS, Oshima A, Yamada Y. 2012. Three-gene predictor of clinical outcome for gastric cancer patients treated with chemotherapy. The Pharmaco Jrnl 12:119-127.

Kumar V, Abbas AK and Fausto N. 2006. Robbins and Cotran Pathologic Basis of Disease, 7th Edition, Reed Elsevier India Pvt. Ltd., New Delhi, India.

Lemon JA, Rollo CD, Boreham DR. 2008. Elevated DNA damage in a mouse model of oxidative stress: impacts of ionizing radiation and a protective dietary supplement. Mutagen 23 (6):473-482.

Mantena SK, Unnikrishnan MK, Uma Devi P. 2008. Radioprotective effect of sulfasalazine on mouse bone marrow chromosomes. Mutagen 23(4):285-29.

Mozdarani H, Nazari E. 2009. Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental γ-irradiation and the influence of vitamin C. Reproduction Res., 137:35-43.

Muller HJ. 1927. Artificial transmutation of the gene. Sci., 66:84-87.

Park K. 2011. Park’s text book of Preventive and Social Medicine. Bhanot Publishers, Jabbalpur. 21:353

Patt HM, Tyree EB, Straube RL, Smith DE. 1949. Cysteine protects against X-irradiation. Sci., 110:213-214.

Snedecor GW, Cochran WG. 1967. Statistical Methods. Iowa St Univ Press, Ames, Iowa, USA,

Suba V, Murugesan T, Bhaskara Rao R, Ghosh L, Pal M, Mandal SC. 2004. Antidiabetic potential of Barleria lupulina extract in rats. Fitoterapia 75(1):1-4.

Sur PK and Manna GK. 1989. A preliminary study of X-ray induced male meiotic chromosome aberration of grasshopper, Oxya velox. Perspective in cytology and genetics (End.- G.K. Manna and U. Sinha). 6:895-899.

Sur PK and Pandey BN. 2004. A comparative study of X-ray and chemical induced marker chromosome aberration in grasshoppers, Oxya velox and Gesonula punctifrons and their alterations by chemicals.
Ph. D. Thesis, Magadh University, Bodh Gaya.

Sur PK, Das PK, Thakur A, Roy S. 2010. A comparative study of X-ray (160r) induced male meiotic chromosome aberrations in two short-horned grasshoppers, Oxya velox and Gesonula punctifrons. Proc Zool Soc India 9(1):79-87.

Trease GE, Evans WC. 1997. Pharmacognosy. Ed.XIV, Mac Millian Publ. Ltd., London.

Wanikiat P, Panthong A, Sujayanon P, Yoosook C, Rossi AG. 2008. The anti-inflammatory effects and the inhibition of neutrophil responsiveness by Barleria lupulina and Clinacanthus nutans extracts. J Ethnopharmacol 116(2):234-244.

Copyright license for the research articles published in Journal of Research in Biology are as per the license given below

Creative Commons License
Journal of Research in Ecology is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0). (www.creativecommons.org)
Based on a work at www.jresearchbiology.com
What this License explains us?

You are free to:

Share — copy and redistribute the material in any medium or format

Adapt — remix, transform, and build upon the material

for any purpose, even commercially.

This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms.

[As given in the www.creativecommons.org website]

Under the following terms:

Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.