Keywords
MS medium
TDZ
Multiple shooting
Acclimatization
How to Cite
Abstract
A protocol for in vitro multiple shoot development and plantlet formation using leaf explant was prescribed for Pergularia daemia (Forssk.) Chiov. medicinally important plant species. Young apical leaf explant was used for callus induction on MS medium containing TDZ and NAA at 1.5 and 0.2 mgl-1 respectively showed maximum callus induction (77%). The amount of callus responded for shoot formation (75%) was obtained in the MS medium containing IBA (1.5 mgl-1) and TDZ (0.3mgl-1). The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.3 mgl-1) for shoots rooted. Regenerated plantlet were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.
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