Keywords
Minimum inhibitory concentration
Kill kinetics
Amphotericin-B
How to Cite
Abstract
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavoniods with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. The flavonoid, flavones and diglycoside (3, 5-dihydroxy flavones, 7-O-b-D-galacturonids-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of Ampotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion these finding suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
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