Keywords
Silver nanoparticles
Characterization
HPLC
How to Cite
Abstract
Nowadays synthesis of nanomaterials by using bio-root is limelight of modern nanotechnology. In the present investigation, we have isolated four strains viz: KFRI 330 (A), KFRI 332 (B), KFRI 351 (C) and KFRI 352 (D) of Beauveria bassiana from the forest soils in Kerala. Spore count was tested for all the strains of B. bassiana stored in the laboratory. Silver nanoparticles were synthesized from the four strains of B. bassiana and the formation of nanoparticles was observed within 48 hours. The synthesized silver nanoparticle has been characterized by UV-Vis spectroscopy, FT-IR and TEM analysis. The appearance of UV-Vis Peak (SPR 440 nm) revealed the reduction of silver metal ions to silver nanoparticles by using the fungal strains. The possible bio-molecules involved in nanoparticles synthesis was identified by HPLC analysis. The functional groups involved in the silver nanoparticles synthesis were identified. The amide group is responsible for the synthesis of silver nanoparticles. From the TEM analysis, the size of the AGNPs has been measured as 4-70 nm (mean 10.7±0.04 nm). It was evident from the HPLC result that primary amines act on capping as a well as a stabilizing agent.
References
Bhainsa Kuber C and D’Souza SF. (2006). Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigates. Colloids and Surfaces B: Biointerfaces 47: 160–164.
Chutao W, Yueqing C, Zhongkang W, Youping Y, Guoxiong P, Zhenlun Li Hua Zhao and Yuxian X. (2007). Differentially-expressed glycoproteins in Locusta migratoria hemolymph infected with Metarhizium anisopliae. Journal of Invertebrate Pathology, 96: 230–236.
Gole, Dash AC, Ramakrishnan V, Sainkar SR, Mandale AB, Rao M and Sastry M. (2001). Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir, 17: 1674–1679.
Ingle A, Gade A, Pierrat S, Sonnichsen C and Rai M. (2008). Myco synthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Current Nanoscience, 4: 141–144.
Jeevan P, Ramya K and Edith Rena A. (2012). Extracellular biosynthesis of silver nanoparticles by culture supernatant of Pseudomonas aeruginosa. Indian Journal of Biotechnology, 11: 72-76.
Kathiresan K, Manivannan S, Nabeel MA and Dhivya B. (2009). Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloid Surface B, 71: 133–137.
Klaus T, Joerger R, Olsson E and Granqvist CG. (1999). Silver-based crystalline nanoparticles, microbially fabricated. Proceedings of National Academy of Science, USA, 96: 13611-13614.
Konishi T, Takeda T, Miyazaki Y, Ohnishi-Kameyama M, Hayashi T, O'Neill MA and Ishii T. (2007). A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose. Glycobiology, 17: 345–354.
Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT and Mohan N. (2010). Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids and Surfaces B: Biointerfaces, 76: 50–56.
Majesh Tomson. (2013). Bioefficacy of Rhynocoris fuscipes Fab. Hemiptera-Reduviidae and Beauveria bassiana Bals.(Ascomycota: Hypocreales) toxic protein and its silver nanoparticles against cotton pests. Ph.D.Thesis., M.S.University, Tirunelveli, Tamilnadu, India. Available in (http://shodhganga.inflibnet.ac.in/handle/10603/39346).
Mouchet F, Landois P, Sarremejean E, Bernard G, Puech P and Pinelli E, Flahaut E and Gauthier L. (2008). Characterisation and in vivo ecotoxicity evaluation of double-wall carbon nanotubes in larvae of the amphibian Xenopus laevis. Aquatic Toxicolology, 87(2): 127-137.
Nair B and Pradeep T. (2002). Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains. Crystal Growth and Design, 2(4): 293-298.
Prabakaran K, Ragavendran C and Natarajan D. (2016). Mycosynthesis of silver nanoparticles from Beauveria bassiana and it’s larvicidal, antibacterial and cytotoxic effect on human cervical cancer (HeLa) cells. RSC Advances, 6: 44972. DOI: 10.1039/c6ra08593h.
Sadowski Z, Maliszewska HI, Grochowalska B, Polowczyk I and Kozlecki T. (2008). Synthesis of silver nanoparticles using microorganisms, Materials Science, 26(2): 419-424.
Sangappa M and Thiagarajan P. (2012). Mycobiosynthesis and characterization of silver nanoparticles from Aspergillus niger: a soil fungal isolate. International Journal of Life Science, Biotechnology and Pharmaceutical Research, 1(2): 282-289.
Willner I, Baron R and Willner B. (2006) . Growing metal nanoparticles by enzymes. Journal of Advanced Materials, 18: 1109-1120.
Copyright license for the research articles published in Journal of Research in Biology are as per the license given below
Creative Commons License
Journal of Research in Ecology is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0). (www.creativecommons.org)
Based on a work at www.jresearchbiology.com
What this License explains us?
You are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material
for any purpose, even commercially.
This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms.
[As given in the www.creativecommons.org website]
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.