Keywords
weightlessness
apoptosis
cell cycle
How to Cite
Abstract
Breast cancer is the most common cancer among women in different societies. Because life is constructed evolutionary on the gravity of ‘g’, removed gravity as a variable can lead to clarify many biologic questions. Today, the weightlessness is a new method to study cellular changes. Weightlessness leads to metabolic and functional changes of the human body, and studies have shown that weightlessness leads to changes in growth and gene expression in cancer cells. The aim of this study is to evaluate the effect of weightlessness on apoptosis and cellular cycle in breast cancer cells. The tests of Annexin-V, PI-flow cytometer, and MTT have been used here. Cells of the weightlessness group are cultured on Clinostat prepared by the United Nations, and in gravity of 0.001g. The cell death and apoptosis using Annexin kit, and cell cycle using the PI and flow cytometer were investigated. Also, the amount of cell damage was determined by MTT. The apoptosis results showed that weightlessness leads to a reduction of 40% in apoptosis in cell line MCF-7, and the increase in BT-20 cell line for two times. Apoptosis in cell line MDA-MB-468 was not affected, and the results showed that the cell cycle and growth in cell line ZR-75 increased at a rate of five times (35% of weightlessness group versus 7% of control). Cell growth in the other categories showed no significant difference between two groups. Also, no significant difference was observed in the amounts of cell damage in groups of weightlessness and control.
References
Antonarakis SE, Krawczak M and Cooper DN. (2000). Disease-causing mutations in the human genome. European Journal of Pediatrics, 159(3): 173-178. DOI: 10.1007/pl00014395.
Arends MJ, Morris RG and Wyllie AH. (1990). Apoptosis. The role of the endonuclease. The American Journal of Pathology, 136(3): 593-608.
Bond M, Murphy G, Bennett MR, Newby AC and Baker AH. (2000). Tissue inhibitor of metalloproteinase-3 induces a Fas-associated death domain-dependent type II apoptotic pathway. Journal of Biological Chemistry. 277(16): 13787-13795. DOI: 10.1074/jbc.m111507200
Clément G and Slenzka K. (2006). Fundamentals of space biology: research on cells, animals, and plants in space. Springer Science and Business Media, 376 p.
Darzynkiewicz Z, Bedner E and Smolewski P. (2001). Flow cytometry in analysis of cell cycle and apoptosis. In Seminars in Hematology, 38(2): 179-193.
Enayatrad M, Amoori N and Salehiniya H. (2015). Epidemiology and trends in breast cancer mortality in Iran. Iranian Journal of Public Health, 44(3):430-1.
Fidler IJ. (2003). The pathogenesis of cancer metastasis: the seed and soil hypothesis revisited. Nature Reviews Cancer. 3(6): 453-458. DOI: 10.1038/nrc1098
Gilberto C, Franchi JR, Cleber S, Moraes Viviane C, Toreti AD, Alexandre E, Nowill and Yong Park K. (2012). Comparison of effects of the ethanolic extracts of Brazilian propolis on human leukemic cells as assessed with the MTT assay. Evidence-Based Complementary and Alternative Medicine, Article ID 918956, 6 p.
Hanahan D and Weinberg RA. (2000). The hallmarks of cancer. Cell, 100(1): 57-70. DOI: 10.1016/s0092-8674(00)81683-9.
Hosseini J, Mahmoodi M, Hakhamaneshi MS, Jalili A, Khoshdel AR, Sheikhfathollahi M, Fakhari S and HosseiniZijoud SM. (2014). Apoptosis effects of aloe-emodin against MCF-7 cell line. Journal of Rafsanjan University of Medical Sciences, 13(1): 41-52.
King RJB and Robins MW. (2006). Cancer biology. 3rd ed. Pearson Education Harlow: Pearson Prentice Hall. 312 p.
Lewis SM. (2014). Medical-surgical nursing: assesment and management of clinical problems. Elsevier Health, 1824 p.
Reddy A and Kaelin WG Jr. (2002). Using cancer genetics to guide the selection of anticancer drug targets. Current Opinion in Pharmacology, 2(4): 366-373.
Mohammadi A and Baradaran B. (2015). Apoptotic effect of the Urtica dioica plant extracts on breast cancer cell line (MDA-MB-468). Journal of Ardabil University of Medical Sciences, 15(3): 283-90.
Thompson CB. (1995). Apoptosis in the pathogenesis and treatment of disease. Science, 267(5203):1456-1462. DOI: 10.1126/science.7878464.
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