Keywords
Medicinal plant
Artemisia annua
Nilgiris
Western Ghats,
India
How to Cite
Abstract
A reliable protocol for callus induction and organogenesis, and successful plantlet survivability through hardening were developed for leaf explants of the medicinal plant species, Artemisia annua L. MS medium containing the auxin, NAA at 0.9mg/l is determined to be the optimum concentration for callus induction. Higher shooting (20.67/callus) was performed in the medium supplemented with BAP and GA3 at 0.5 and 1.0 mg/l respectively while subculturing the callus. Maximum number of roots (12.00 roots/shoot) was noted to be obtained in the medium containing IBA at 0.9mg/l. The in vitro regenerated plantlets were successfully acclimatized (86% survivability rate) in the hardening medium encomposing vermiculate, coir waste and forest litter in the ratio of 1:1:1 by volume
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