Abstract
DNA barcode technique has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. The present study carried out for the barcode sequences for identification of the Plectropomus leopardus. The results of shorter sequence 113bp of Plectropomus leopardus is effective to identify a specimen and confirm up to a species level. This sequence also exhibited deep interspecific divergences that allowed for efficient discrimination among the Grouper species. This mini barcode sequence technique might be used efficiently to distinguish Plectropomus leopardus with minimal expenditure and efforts. These results also support that formalin fixation samples can also be used for DNA molecular taxonomy
References
Chen EY, Seeburg PH. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 2:165-170.
Fakhrai-Rad H, Pourmand N, Ronaghi M. 2002. Pyrosequencing: an accurate detection platform for single nucleotide polymorphisms. Human Mutation 19:479-485.
Hajibabaei M, Gregory A, Singer C, Hickey DA. 2006. Benchmarking DNA Barcoding: an assessment using available primate sequences. Genome 49:851-854. Doi:10.1139/G06-025.
Hajibabaei H, DeWard JR, Ivanova NV, Ratnasingham S, Dooh RT, Kirk SL, Mackie PM, Hebert PDN. 2005. Critical factors for assembling a high volume of DNA barcodes. Phil. Trans. R. Soc. B. 10:1-9.
Hebert PDN, Cywinska A, Ball SL, DeWard JR. 2003a. Biological identifications through DNA barcodes. Proc. R. Soc. Lon. B. 270: 313-321. Doi 10. 1098/rspb. 2002.2218.
Hebert PDN, Ratnasingham S, DeWard JR. 2003b. Barcoding animal life: cytochrome c oxidase subunit1 divergences among closely related species. Pro. Royal Soc. Lon. B. 270:S96-S99.
Heemstra PC, and Randall JE. 1993. Groupers of the World. FAO Fisheries Synopsis. 16:1-125.
James PSBR, Murthy VS, Nammalwar P. 1996. Groupers and snappers of India: biology and exploitation. In: Biology, Fisheries and Culture of Tropical Groupers and Snappers, Arreguin-Sanchez F, Munro JL, Balgos MC, Pauly D. (Eds.). ICLARM Conf. Proc. 48:106-136.
Sambrook J, Fritsch EF, Maniatus T. 1989.Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor Laboratory press, Cold Spring harbour, NY.13.
Sanger F, Nicklen S, Coulson AR. 1977. DNA Sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA. doi:10.1073/pnas.74.12.5463. 74:5463-5467.
Schander C, Kenneth HM. 2003. DNA, PCR and formalinized animal tissue - a short review and protocols. Organisms Diversity and Evolution 3:195-205.
Smith MA, Fisher BL, Hebert PDN. 2005. DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar. Phil. Trans. R. Soc. Lon. B., 360:1825-1834.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24:1596-1599.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species, Phil. Trans. R. Soc. Lon. B., 360:1847-1857.
Copyright license for the research articles published in Journal of Research in Biology are as per the license given below
Creative Commons License
Journal of Research in Ecology is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0). (www.creativecommons.org)
Based on a work at www.jresearchbiology.com
What this License explains us?
You are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material
for any purpose, even commercially.
This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms.
[As given in the www.creativecommons.org website]
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.