Keywords
toxicity
enzyme inhibition
wound isolates
phytochemicals, and bacterial response
How to Cite
Abstract
Inhibition of total dehydrogenase enzyme activity in pathogenic gram positive and gram negative micro organisms exposed to methanol extract of Chromolaena odorata was used as an index for assessment of its antimicrobial activity. Assay of total dehydrogenase enzyme activity was done in the test organisms (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli) using 2,3,5-triphenyltetrazolium chloride (TTC) as an artificial electron acceptor which was reduced to the red-coloured triphenyl-formazan. Response of the bacterial isolates varied with extract concentration. Dehydrogenase activity was progressively inhibited in a logistic dose-response fashion. The gram negative Escherichia coli responded more markedly than Pseudomonas aureginosa and gram positive Staphylococcus aureus. Inhibitory concentrations (IC50) of the methanol extract against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were 208.49 μg/ml, 1361.01 μg/ml, and 903.08 μg/ml respectively. Preliminary phytochemical screening of the extract gave positive reactions for alkaloids, flavonoids, tannins, 4-hydroxybenzoic acid, and glycosides. These phytochemicals may be responsible for the observed inhibition of total dehydrogenase enzyme activity that translates to antimicrobial action in these pathogenic organisms
References
Alisi CS and Onyeze GOC. 2008. Nitric oxide scavenging ability of ethyl acetate fraction of methanolic leaf extracts of Chromolaena odorata (Linn.). Afr. Journ. Bioch. Res. 2 (7):145-150.
Alisi CS and Onyeze GOC. 2009. Biochemical mechanisms of wound healing using extracts of chromolaena odorata-linn* Nig. Journ. Bioch and Mol. Biol. 24(1):22-29.
Alisi CS, Nwanyanwu CE, Akujobi CO and Ibegbulem CO. 2008. Inhibition of dehydrogenase activity in pathogenic bacteria isolates by aqueous extracts of Musa paradisiaca (Var Sapientum). Afr. Journ. Biotech. 7(12):1821-1825.
Aziz NH, Frag SE, Mousa LA and Abo-Zald MA. 1998. Comparative antimicrobial and antifungal effects of some phenolic compounds. Microbios 93(374):43-54.
DelRío A, Obdulio BG, Castillo J, Marin RR, and Ortuno A. 1997. Uses and properties of citrus flavonoids. J. Agric. Food Chem. 45:4505-4515.
Evans WC. (ed) 2002. Trease and Evans pharmacognosy: Edinburg: W.B.Saunders. Holme GLR, Plucknet DL, Pancho JV and Herberger JP. 1977. Chromolaena odorata, The World’s Worst Weeds: Distribution and Biology :The University Press of Hawaii.
Le TT. 1995. The 5th European Tissue Repair Society Annual Meeting, Padova, Italy, (Abst. 30). Liu RH. 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 134:3479S- 3485S.
Nweke CO, Okolo JC, Nwanyanwu CE and Alisi CS. 2006. Response of planktonic bacteria of new Calabar river to zinc stress. Afr. Journ. Biotechnol 5 (8):653-658.
Nweke CO, Alisi CS, Okolo, JC and Nwanyanwu CE. 2007. Toxicity of Zinc to heterotrophic Bacteria from a tropical river sediment. Applied Ecology and Environmental Research 5(1): 123-132.
Nwogu LA, Alisi CS, Ibegbulem CO and Igwe CU. 2007. Phytochemical and antimicrobial activity of ethanolic extract of Landolphia owariensis leaf. Afr. J. Biotechnol 6(7):890-893.
Nwogu LA, Alisi CS, Igwe CU and Ujowundu CO. 2008. A comparative study of the antimicrobial properties of the ethanolic extracts of Landolphia owariensis leaf and root. Afr. J. Biotechnol 7(4):368-372.
Marquardt DW. 1964. An algorithm for least squaresestimation of non-linear parameters.J.Soc.Ind.Appl.Math. 2:431-441.
Oberbremer A and Muller H. 1989. Aerobic stepwise hydrocarbon degradation and formation of biosurfactants by an original soil population in a stirred bioreactor. Appl. Microbiol. Biotechnol. 31:582-586.
Okwu DE. 2004. Phytochemical and vitamin content of indigenous spices of South Eastern Nigeria. J. Sustain. Agric. Environ. 6:30-34.
Phan TT, Wang L, See P, Grayer RJ, Chan SY and Lee ST. 2001. Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage: implication for cutaneous woundhealing. Biol. Pharm Bull. 24(12):1373- 1379.
Raza H and John A. 2007. In vitro protection of reactive oxygen species-induced degradation of lipis, proteins and 2-deoxyribose by tea catechins. Food and Chem. Toxicol. 45:1814-1820.
Salah W, Miller NJ, Pagauga G, Bolwell GP, Rice E and Evans C. 1995. Polyphenolic flavonoids as scavenger of aqueous phase radicals and chain breaking antioxidants. Arch. Biochem. Biol. 2:339-346.
Tabatabai MA. 1982. Soil enzymes. In: A.L. Page, R.H. Miller and D.R. Reeny (eds.). Methods in Soil Analysis, Part 2. Wisconsin, American Society of Agronomy, Soil Science Society of America 903-947.
Tortensson L. 1997. Microbial assays in soil. In: J. Tarradellas, G. Bitton and D. Rossel (eds). Soil Ecotoxicology. CRC Lewis Publishers. Boca Raton 207-233.
Von Mersi W and Schinner F. 1991. An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol. Fertil. Soils 11:216-220.
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