Evaluation of Antibacterial activity of Medicinal Plants on Fish Pathogen Aeromonas hydrophila
Abstract
This study evaluated the antimicrobial potency of aqueous extract of three common medicinal herbs, Azadirachta indica (Neem leaves), Solanum torvum (Sundakai fruit coat) and Curcuma longa (Turmeric,rhizome) against the in vitro growth of pathogenic bacterium Aeromonas hydrophila isolated from infected fresh-water fish, Channa striatus was assessed by disc diffusion assay.
Determination of minimum inhibitory concentration (MIC) for each aqueous herbal extract was done on Muller Hinton agar swabbed with 0.5 ml of overnight culture of A. hydrophila (106cfu/ml). After incubation period, zones of inhibition around herbal extracts incorporated discs were measured.
The strongest antibacterial activities among all plant species were obtained by the aqueous extract of A. indica with inhibition zone of 18 mm against A. hydrophila. S. torvum demonstrated moderate (11 mm) and C. longa marked weak (8 mm) inhibiting activity against A. hydrophila.
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