Comparison of horseradish peroxidase, soybean peroxidase and radish peroxidase for the polymerization of phenol
Abstract
Polymerization of Phenol was carried out in laboratory using peroxidase enzyme extracted from three different plant sources namely, horseradish roots, soybean hulls and radish roots. The ambient room temperature during the study period was between 27-32oC. Phenol concentration of 100 - 300 mg/L was given to the free enzymes. Phenol polymerization of 84% was achieved at 100 mg/L phenol using free HRP enzyme. Similarly, 72% and 76% phenol polymerization was obtained using free SBP and RP respectively at 100 mg/L phenol. Peroxidase isolated from horseradish roots was able to polymerize phenol more efficiently than the enzymes isolated from soybean hulls and radish roots. As the concentration of phenol increased, reduction in phenol polymerization efficiency was observed for all the three enzymes
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