Preliminary Phytochemical Analysis and Antimicrobial Activity of Some Weeds collected from Marathwada Region
Abstract
The aqueous and ethanolic extracts of selected weeds such as Portulaca oleracea L. (Portulaceae), Cardiospermum helicacabum L. (Sapindaceae), Euphorbia hirta L. (Euphorbiaceae), Crotalaria retusa L. (Fabaceae) and Euphorbia heterophylla L. (Euphorbiaceae) were screened for phytochemical analysis and antimicrobial activity. The antimicrobial activities were tested against two gram-positive bacteria (Bacillus subtilis, Staphylococus aureus), one gram-negative bacterium (Pseudomonas aeruginosa) and a mould Aspergillus niger by agar diffusion method. The phytochemicals like alkaloids, saponins, tannins, cardiac glycosides, steroids and flavonoids are investigated from plant samples. Tannins, steroids and flavonoids were absent in the Cardiospermum helicacabum L. Remarkable antibacterial activity was observed in the aquous and ethanolic (root and leaves) extracts of Portulaca oleracea L. while Cardiospermum helicacabum L. showed no results in same experimental conditions as compared with standard antibiotics. The weed Euphorbia hirta L possess significant antifungal activity as compared to nystatin used as a standard antifungal agent
References
Ambasta SP. 1992. The useful plants of India. Publications and Information Directorate. CSIR, New Delhi, India.
Bongoh, Moochang and Jun Hwang. 2000. Detection of antifungal activity in Portulaca oleracea by a single cell bioassay system” Phytotherapy research 14(5):329-332.
Jigna Parekh, Sumitra V, Chanda. 2007. In vitro Antimicrobial activity and Phytochemical Analysis of Some Indian Medicinal plants, Turk. J. Biol 53- 58.
Leung AY and Foster S. 1996. Encyclopedia of Common Natural Ingredients used in food, Drug and cosmetic, 2nd Ed. New York, John Wiley & Sons. Inc.
Lwu MW, Duncan AR, Okunji CO. 1999. New antimicrobials of plant origin. In: Janick J. ed. Perspectives on New Crops and New Uses. Alexandria, VA: ASHS Press 457-462.
Naik VN. 1998. The Flora of Marathwada Amrut Prakashan, Aurangabad, M.S. (India). Vol I and II.
Newman FI and Andrews RC. 1973. Allelopathy among some British grassland species II. Influence of soaked exudates on phosphorus uptake. J.Ecol.65:399-411.
Prajapati ND, Kumar U.2003. Agro’s Dictionary of Medicinal plants. Agrobios (India), Jodhpur 96.
Rabe Tandvan Staden J. 2000. Isolation of an antimicrobial sesquiterpenoid from Warbugie salutaris. J. Ethnopharcol 93:171-174.
Ratnam RK and Martin P. 2006. Ethnomedicinal plants.Agrobios (India) publication, Jodhpur 227.
Rodriguez E, Twers GHN, Mitchell JC. 1976. Biological activites of sesquiterpene Lactones. Phytochemistry 15:1573.
Sahoo S, Kar DM, Mohapatra S, Raut SP and Dash SK. 2006. Antibacterial activity of Hybanthus enneaspermus against selected UTI pathogens. Ind. J. Pharmacol. Soc 68(5):653-655.
William D, Warden CJH and Hooper D. 2005. “Pharmacographia india” A History of the principal Drug of Vegetable Origin. Srishti publication, New Delhi 366-367.