Lignolytic and phosphate solubilizing efficiency of fungal species isolated from Arecanut husk waste
Abstract
A wide range of microorganisms are capable of degrading lignin present in the Arecanut husk waste for their growth. The present study has been undertaken to isolate lignolytic and phosphate solubilizing fungi from Arecanut husk waste. Isolation of fungi by using serial dilution method and direct soil plate method was employed. Estimation of lignolytic and phosphate solubiling efficiency of isolated fungi was carried out. Fifteen varieties of fungal species were isolated from the Arecanut husk waste. Of these fifteen fungal species isolated seven showed lignolytic property and nine showed phosphate solubilizing activity. The lignolytic ability shows that the zone of clearance was higher in Gibberella fujikuroi (0.8 ± 0.02 cms), medium in Aspergillus niger (strain-1) (0.6 ± 0.06 cms) and very low activity in Aspergillus flavus (0.2 ± 0.02 cms). The Phosphate solubilizing ability shows that the zone of clearance was higher in Aspergillus terreus (0.8 ± 0.03 cms), medium in Botrytis cinerea (0.5 ± 0.08 cms) and very low activity in Aspergillus niger (Strain-2) and Unidentified-3 (0.1 ± 0.03 cms). The Phosphate solubilizing activity was higher in Unidentified-2 (550 ± 8.50 μg/ml), medium in Unidentified-1 (530 ± 10.00 μg/ml) and very low activity in Aspergillus niger (strain-2) (40 ± 2.52 μg/ml). Total biomass yield of the fungi showed the highest rate of growth for Aspergillus niger (strain-1) (0.31 ± 0.02 gm/days), medium growth rate in unidentified-2 (0.29 ± 0.01 gm/days) and very low growth rate in unidentified-1 (0.06 ± 0.01 gm/days) in twelve days incubation. An effective fungal Species is very essential for the degradation of lignocellulosic wastes and municipal solid wastes
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