Bioremediation of oil contaminated soil using biosurfactant produced by Pseudomonas aeruginosa

Pradeep NV; Anupama; Anitha G; Renukamma; Sudhakar  Avvaru; Afreen SS

Pradeep NV Assistant Professor, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India
Anupama Assistant Professor, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India
Anitha G B.E Scholar, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India
Renukamma B.E Scholar, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India
Sudhakar Avvaru B.E Scholar, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India
Afreen SS B.E Scholar, Department of Biotechnology, Ballari Institute of Technology and Management, Bellary, India

Keywords: Biosurfactant, Solid state fermentation, Bioremediation, Pseudomonas aeruginosa, Surface tension

Abstract

Biosurfactants are surfactants that are biologically produced from bacteria, yeasts and fungi. Examples include Pseudomonas aeruginosa which produce biosurfactants from various substrates including sugars, oil and wastes. Biosurfactants have the property of reducing surface and interfacial tension. These are biodegradable and nontoxic. In the present study saw dust and rice husk were used as substrates for biosurfactant production. Pseudomonas aeruginosa was isolated from contaminated soil sample collected from diesel contaminated site for the production of Biosurfactant. The fermentative production of biosurfactant from Pseudomonas aeruginosa was carried out by solid state fermentation (SSF) using two low cost substrates (rice husk and saw dust). These substrates were inoculated with P. aeruginosa and surface tension was measured on 1st, 3rd, 5th, 7th and 9th day. The highest reduction of surface tension (22.9 mN/m) was obtained using saw dust on 7th day. Produced biosurfactant have been tested for remediation of oil contaminated soil. The contaminated soil was prepared in the laboratory by mixing oil, sand and seed (diesel contaminated soil) in the ratio of 10:2:1. Contaminated soil was further transferred to containers and was allowed for acclimatization. Three containers were used in this study 1) Containing contaminated soil without biosurfactant (C1). 2) Container containing contaminated soil with 4 g of biosurfactant per kg of soil (C2). 3) Containing contaminated soil with 8 g biosurfactant per kg of soil (C3). The oil remaining in soil was determined by solvent extraction method using n-hexane. Oil removal was high in the C2 container which contained 4 g of biosurfactant per kg of soil.

References

Bento FM, Camargo FA, Okeke BC, Frankenberger WT. 2005. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiological Research. 160:249-255.

Calvo C, Manzanera M, Silva-Castro GA, Uad I, Gonzalez-Lopez J. 2009. Application of bioemulsifiers in soil oil bioremediation processes, Future prospects. Science of the total Environment. 407:3634-3640.

Das P, Mukherjee S, Sen R. 2009. Substrate dependent production of extracellular biosurfactant by a marine bacterium. Bioresource Technology. 100:1015-1019.

Dubey K and Juwarkar A. 2001. Distillery and curd whey wastes as viable alternative sources for biosurfactant production. World Journal of Microbiology & Biotechnology. 17:61-69.

George S and Jayachandran K. 2008. Analysis of Rhamnolipid Biosurfactants Produced through Submerged Fermentation Using Orange Fruit Peelings as Sole Carbon Source. Applied Biochemistry and Biotechnology. 158(3):694-705.

Ligia Rodrigues, Ana Moldes, Jose Teixeira, Rosario Oliveira. 2006. Kinetic study of fermentative biosurfactant production by Lactobacillus strains. Biochemical Engineering Journal. 28:109-116.

Liu PWG, Whang LM, Yang MC, Cheng SS. 2008. Biodegradation of diesel-contaminated soil: A soil column study. Journal of the Chinese Institute of Chemical Engineers. 39:419-428.

Lu M, Zhang Z, Sun S, Wang Q, Zhong W. 2009. Enhanced degradation of bioremediation residues in petroleum-contaminated soil using a two-liquid-phase bioslurry reactor. Chemosphere.77:161-168.

Margesin R. 2000. Potential of cold-adapted microorganisms for bioremediation of oil-polluted Alpine soils. International Biodeterioration & Biodegradation. 46:3-10.

Mulligan CN. 2000. Environmental applications for biosurfactants. Environmental Pollution. 133:183-198.

Scheibenbogen K, Zytner RG, Lee H, Trevors JT. 1994. Enhanced removal of selected hydrocarbons from soil by Pseudomonas aeruginosa UG2 biosurfactants and some chemical surfactants. Journal of Chemical Technology & Biotechnology. 59:53-59.

Turkovskaya OV, Dmitrieva TV and Muratova A, Yu A. 2001. Biosurfactant-Producing Pseudomonas aeruginosa Strain. Applied Biochemistry and Microbiolog., 37 (1):71-75.

Urum and Pekdemir T. 2004. Evaluation of biosurfactants for crude oil contaminated soil washing. Chemosphere. 57:1139-1150.

Urum K, Pekdemir T and Gopur M. 2003. Optimum conditions for washing of crude oil-contaminated soil with biosurfactant solutions. Institution of Chemical Engineers Trans IChemE., 81, Part B.

Veenanadig NK, Gowthaman MK, Karanth NGK. 2000. Scale up studies for the production of biosurfactant in packed column bioreactor. Bioprocess Engineering. 22:95-99.

Whang LM, Liu PWG, Maa CC, Cheng SS. 2008. Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. Journal of Hazardous Materials. 151:155-163.

Youssef NH, Duncana KE, Naglea DP, Savagea KN, Knapp RM, McInerneya MJ. 2004. Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods.56: 339-347.

Zhang G, Wu Y, Qian X, Meng Q. 2005. Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids. Journal of Zhejiang University Science.6 B (8):725-730.

Bioremediation of oil contaminated soil using biosurfactant produced by Pseudomonas aeruginosa

Abstract

References

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