Cell surface properties of Phenol-Utilizing Bacteria Isolated from petroleum refinery wastewater
Keywords:
Phenol-utilizing bacteria, Hydrophobicity, SAT, Congo red binding
Abstract
Cell surface hydrophobicity of six phenol-utilizing bacteria isolated from Port Harcourt Petroleum refinery wastewater was assessed via bacterial adhesion to hydrocarbon (BATH), salt aggregation test (SAT) and Congo red binding (CRB) assays. The test organisms exhibited high to moderate hydrophobicity with BATH assay respectively when n-octane and p-xylene were employed. Bacillus sp. RBD, Escherichia coli. OPWW, Corynebacterium sp. DP, Citrobacter sp. RW and Pseudomonas sp. SD showed moderate hydrophobicity in SAT assay. On the other hand, Pseudomonas sp. RWW showed high hydrophobicity in SAT assay. Similar results of moderate hydrophobicity were obtained with CRB except Corynebacterium sp. DP that exhibited high hydrophobicity value of 14.70±1.00μg. The results obtained in this study showed that the isolates are mainly moderately hydrophobic which make them good candidates in the clean up activity of organic pollutants in polluted sites.
References
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Rozgonyi F, Szitha KR, Ljungh A, Baloda SB, Hjerten S, Wadstrom T. 1985. Improvement of the salt aggregation test to study bacterial cell surface hydrophobicity. FEMS Microbiol. Lett. 30:131-138.
Sarala TD, Sabitha MA. 2012. Water quality and environmental assessment of sugar mill effluent. J. Res. Biolo., 2: 125 - 135.
Sorongon ML, Bloodgood RA, Burchard RP. 1991. Hydrophobicity, adhesion and surface-exposed proteins of gliding bacteria. Appl. Environ. Microbiol., 57:3193-3199.
Soto-Rodriguez SA, Roque A, Lizarraga-Partida ML, Guerra-Flores AL, Gomez-Gil B. 2003. Virulence of luminous vibrios to Artemia franciscana nauplii. Dis Aquat Org., 53:231-240
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Zhang Y, Miller RM. 1994. Effect of Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane. Appl. Environ. Microbiol., 60:2101-2106.
A.P.H.A. 1985. Standard Methods for the examination of Water and Wastewater, 16th edition. American Public Health Association, American water works Association and Water Pollution control Federation, Washington, D.C.
Basson A, Fleming LA, Chenia HY. 2008. Evaluation of adherence, hydrophobicity, aggregation and biofilm development of Flavobacterium johnsoniae-like isolates. Microb. Ecol., 37:1-14.
Coelho A, Castro AV, Dezotti M, Sant’Anna GL. 2006. Treatment of petroleum refinery sourwater by advanced oxidation processes. J. Hazard. Mat., B137: 178-184.
Folsom BR, Chapman PJ, Pritchard PM. 1990. Phenol and trichloroethylene degradation by Pseudomonas cepcia GA: Kinetics and interaction between substrates. Appl. Environ. Microbiol., 56:1279-1285.
Haider K, Azad AK, Qadri F, Nahar S, Ciznar I. 1990. Role of plasmids in virulence-associated attributes and in O-antigen expression in Shigella dysentriae type 1 strain. J. Med. Microbiol., 33:1-9.
Hill GA, Robinson CW. 1975. Substrate inhibition kinetics: Phenol degradation by Pseudomonas putida. Biotech. Bioeng., 17:1599-1615.
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. 1994. Bergey’s Manual of Determinative Bacteriology, 9th ed. Williams, Wilkins. Baltimore.
Kaczorek E, Chrzanowski Ł, Pijanowska A, Olszanowski A. 2008. Yeast and bacteria cell hydrophobicity and hydrocarbon biodegradation in the presence of natural surfactants: rhamnolipides and saponins. Biores. Technol., 99:4285-4291.
Krishnakumar PK, Dineshbabu AP, Sasikummar G, Bhat GS. 2007. Toxicity of treated refinery effluentt using brine Shrimp (Artemia salina) egg and larval bioassay. Fishery Technol., 44:85-92.
Lachica RV, Zink DL. 1984. Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica. Infect. Immun., 44:540-543.
Lee KK, Yii KC. 1996. A comparison of three methods for assaying hydrophobicity of pathogenic Vibrios. Lett. Appl. Microbiol., 23:343-346.
Leung YM, Ou YJ, Kwan CY, Loh TT. 1997. Specific interaction between tetrandrine and Quillaja saponins in promoting permeabilization of plasma membrane in human leukemic HL-60 cell. Biochimica et Biophysica Acta., 1325:318-328.
Lindahl M, Faris A, Wadstrom T, Hjerten S. 1981. A new test based on salting out to measure relative surface hydrophobicity of bacterial cells. Biochim. Biophys. Acta 677:471-476.
Majtan V, Majtanova L. 2001. In vitro effect of fluoroquinolones and aminoglycosides on the surface hydrophobicity and motility of Salmonella enterica serotype Typhimurium DT104. Biologia Bratislava. 56(6):625-631.
Mattos-Guaraldi AL, Formiga LCD, Andrade AFB. 1999. Cell surface hydrophobicity of sucrose fermenting and nonfermenting Corynebacterium diphtheriae strains evaluated by different methods. Curr. Microbiol., 38:37-42.
Mo ZL, Wang XH, Yu Y, Li HR, Ji WS, Xu HS. 2000. Selection of organic pollutants degrading bacteria in shrimp ponds. J. Fish. Chin., 24:334-338.
Noweco Laboratory. 1997. Norwalk Wastewater Equipment Company, Inc. 220 Republic Street Norwalk, Ohio U.S.A. 44857-1156. http://www.norweco.com/htmL/lab/WhatTests.htm.
Nwanyanwu CE, Abu GO. 2010. In vitro effects of petroleum refinery wastewater on dehydrogenase activity in marine bacterial strains. Ambi. Agua 5:21-29.
Okerentugba PO, Ezeronye OU. 2003. Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria. Afr. J. Biotechnol. 2 (9):288-292.
Payne SM, Finkelstein RA. 1977. Detection and differentiation of iron-responsive avirulent mutants on congo red agar. Infect. Immun., 18:94-98.
Pijanowska A, Kaczorek E, Chrzanowski L, Olszanowski A. 2007. Cell hydrophobicity of Pseudomonas sp. and Bacillus sp. bacteria and hydrocarbon biodegradation in the presence of Quillaya saponin. World J Microbiol Biotechnol. 23:677-682.
Qadri F, Hossan SA, Ciznar I, Haider K, Ljungh A, Wadstrom T, Sack DA. 1988. Congo red binding and salt aggregation as indicator of virulence in Shigella species. J. Clin. Microbiol., 26:1343-1348.
Rosenberg M. 1981. Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity. Appl. Environ. Microbiol., 42:375-377.
Rosenberg M. 1984. Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity. FEMS Microbiol. Lett., 22:289-295.
Rosenberg M, Kjelleberg S. 1986. Hydrophobic interactions: Role in bacterial adhesion. Adv. Microbiol. Ecol., 9:353-393.
Rozgonyi F, Szitha KR, Ljungh A, Baloda SB, Hjerten S, Wadstrom T. 1985. Improvement of the salt aggregation test to study bacterial cell surface hydrophobicity. FEMS Microbiol. Lett. 30:131-138.
Sarala TD, Sabitha MA. 2012. Water quality and environmental assessment of sugar mill effluent. J. Res. Biolo., 2: 125 - 135.
Sorongon ML, Bloodgood RA, Burchard RP. 1991. Hydrophobicity, adhesion and surface-exposed proteins of gliding bacteria. Appl. Environ. Microbiol., 57:3193-3199.
Soto-Rodriguez SA, Roque A, Lizarraga-Partida ML, Guerra-Flores AL, Gomez-Gil B. 2003. Virulence of luminous vibrios to Artemia franciscana nauplii. Dis Aquat Org., 53:231-240
Walsh SE, Maillard JY, Rusell AD, Catrenich CE, Charonneau DL, Bartolo RG. 2003. Activity and mechanism of action of selected biocidal agents on gram positive and gram negative bacteria. J. Appl. Microbiol., 94:240-247.
Zhang Y, Miller RM. 1994. Effect of Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane. Appl. Environ. Microbiol., 60:2101-2106.
Published
2012-06-08
How to Cite
CE, N., CS, A., CO, N., & JC, O. (2012). Cell surface properties of Phenol-Utilizing Bacteria Isolated from petroleum refinery wastewater. Journal of Research in Biology, 2(4), 383-391. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/229
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