Studies on some biochemical effects of aqueous leaf Extract of landolphia owerrience on albino rats
Keywords:
Ethno-medicine, hemoglobin, liver enzymes, medicinal plant, peroxidation, protein
Abstract
The effects of aqueous leaf extract (crude) of Landolphia owerrience on some biochemical parameters in adult male and female Albino rats were investigated. Administration of the plant leaf extract was by gavage at a dose level of 20 mg/kg, 30 mg/kg and 40 mg/kg body weight in 0.5 ml saline respectively, daily for 21 days. The concentration of ALT and AST were non-significantly increased as the dose increased. Alkaline phosphatase (ALP) level also increased non-significantly, but not dose dependent. Protein level also significantly increased in a dose dependent fashion. Hemoglobin concentration increased significantly, while lipid peroxidation level decreased non-significantly as the dose increased. The non-significant increase in liver enzyme concentrations in this study showed that the Landolphia owerrience leaf extract may not be toxic to the rat liver, while the low lipid peroxidation levels showed that it may have antioxidant activity.
References
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Tindal HD. 1965. Fruits and vegetables of West Africa. (1st Edition). Food and Agriculture Organization, USA
Wallin B, Rosengren B, Shertzer HG and Camejo G. 1993. Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtiter plate: its use for evaluation of antioxidants Anal. Biochem., 208:10-15.
Weber LW, Boll M and Stampfl A. 2003. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit. Rev. Toxicol., 23:105-136.
Alada ARA. 2000. The haematological effect of Telfairia occidentalis diet preparation. Afr. J.Biomed. Res: 185-186.
Burkill HM. 1994. The useful plants of tropical West Africa. (2nd edition). Royal Botanic Gardens, Kew, ISBN: 0947643567
Cai Q, Rahan RO and Zang R. 1997. Dietary flavonoids, quercetin, luteolin and genistein, reduce DNA damage and lipid peroxidation and quench free radicals. Cancer Lett. 119:99-108
Chang JS, Chiang LC, Hsu FF and Lin CC. 2004. Chemoprevention against hepatocellular Carcinoma of Comus officinalis - invitro. Am. J. Chin. Med., 32(5):717-725
Dacie JV and Lewis SM. 1990. Practical Hematology. Churchill Livingstone New York 34.
Ebi GC and Ofoefule SI. 1997. Investigation into the folkloric antimicrobial activities of Landolphia owerrience. Phyt. Res., 11:149-151.
Effraim KD, Jacks TW and Sodipo OA. 2003. Histopathological studies on the toxicity of Ocimum gratissimum on some organs of Rabbits: African J. Biomed., Res. 6:21-25.
Hsieh YS, Kuo WH, Lin TW, Chang HR, Lin TH, Chen PN and Chu SC. 2009. Protective effects of Berberine against Low-Density Lipoprotein (LDL) oxidation and oxidized LDL- Induced cytotoxicity on endothelial cells. J Agric Food Chem., 55:10437-1044
Hwang JM, Wang CJ, Chou FP, Tseng TH, Hsieh YS, Lin WL and Chu CY. 2002. Inhibitory effect of berberine on tert-butyl hydroperoxide-induced oxidative damage in rat liver. Arch Toxicol., 76:664-670.
Iweala EEJ and Obidoa O. 2010. Studies on Some Biochemical and Histological Changes Associated with Long Term Consumption of Leaves of Ocimum gratissimum L. in Male Rats. Am. J. Food Technol., 5:376-384
Iwu MM. 1993. Handbook of African Medicinal Plants. CRC Press, Boca Raton, Ann. Arbor and London, Tokyo.
Jensen JE and Freese D. 2009. Liver Function Tests. Colorado Center for Digestive disorders 205s.Suite A Longmont Co. 80501. 151-163.
Koba K, Akahoshi A, Yamasaki M, Tanaka K, Yamada K, Iwata T, Kamegai T, Tsutsumi K and Sugano M. 2002. Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats. Lipids, 37:343-350.
Lehninger AL, Nelson OL and Cox MM. 2005. Text Book of Biochemistry. (6th edition) Worth Publishers. NY USA 450.
Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193:265-275
Malomo SO. 2000. Toxicological implication of Ceftriaxome administration in rats. Nig. J. Biochem. Mol. Biol., 15(1):33-35
Miller NJ and Ruiz-Larrea MB. 2002. Flavonoid and other plant phenols in diet: Their significance as antioxidants. J. Nutr. Environ. Med., 12:39-51.
Mohana Sundaram, Sivakumar Karthikeyan, Magesh Gopinath, Sheeladevi Thirumalai, and Pennarasi Prasanna. 2011. Studies on phytochemicals, antibacterial and Antioxidant effects of leaf extracts of lamprachaenium microcephalum.. Journal of Research in Biology 4: 279-284.
Mohd-Esa N, Hern FS, Ismail A and Yee CL. 2010. Antioxidant activity in different parts of roselle (Hibiscus sabdariffa) extracts and potential exploitation of their seeds. Food Chem., 122:1055-1060.
Neuman HV and Vreedenal M. 1967. An improved alkaline phosphatase determination with p-nitrophenol phosphate. Clinica Chimica Acta.17(2):183-187.
Oboh G. 2005. Hepatoprotective property of ethanolic and aqueous extracts of Telfairia occidentalis (Fluted pumpkin) leaves against garlic-induced oxidative stress. J. Med. Food 8:560-563.
Okeke MI, Iroegbu CU, Eze EN, Okoli AS and Esimone CO. 2001. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J. Ethnopharmacol., 78(2-3):119-27
Person JGM, Dilst FJH, Kuijpers RP, Leenwerberg AJM, Vonk GJA and Van-Dilst FJH. 1962. The African species of Landolphia. P.Beaux Series of Revisions of Apocynaceae. Xxxiv Wageningen Agricultural University Paper No.92. 323.
Ranjna O. 1996. Practical Clinical Biochemistry, Methods and Interpretations (2nd edition) Jaypee Brothers Medical Publishers. India 100-109
Reitman S and Frankel SL. 1957. Determination of ALT and AST concentrations in serum. Am. J. Chem Path., 28:56
Ross JA and Kasum CM. 2002. Dietary flavonoids: Bioavailability, metabolic effects and safety. Ann. Rev. Nutr., 22:19-34
Roy CK, Kamath JV and Asad M. 2006. Hepatoprotective activity of Psidium guajava Linn leaf extract. Ind. J. Exp. Biol., 44:305-311
Subashree B, Baskar R, Laxmi KR, Lijina SR and Rajasekaran P. 2009. Evaluation of antioxidant potential in selected green leafy vegetables. Food Chem., 115:1213-1220.
Tindal HD. 1965. Fruits and vegetables of West Africa. (1st Edition). Food and Agriculture Organization, USA
Wallin B, Rosengren B, Shertzer HG and Camejo G. 1993. Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtiter plate: its use for evaluation of antioxidants Anal. Biochem., 208:10-15.
Weber LW, Boll M and Stampfl A. 2003. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit. Rev. Toxicol., 23:105-136.
Published
2012-06-16
How to Cite
Uhegbu, F. O., Ugbogu, A. E., & Nwoku, K. C. (2012). Studies on some biochemical effects of aqueous leaf Extract of landolphia owerrience on albino rats. Journal of Research in Biology, 2(5), 410-417. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/232
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