Effect of extract of Bacillus megaterium 9554 on oral cancer cell line SCC131
Keywords:
Apoptosis, Flow cytometry, Bacillus, Cancer
Abstract
A microbe, identified as Bacillus megaterium 9544 was isolated from the soil sample collected from Manali, Himachal Pradesh. The bacterial broth extract had the presence of compound(s) that induced apoptotic like features on oral cancer cells, like morphological changes, condensation of nucleus and detachment of cancer cells from the surface of culture vessel. The extract did not cause any kind of changes in mitochondrial membrane potential as evidenced from flow cytometric analysis and did not cause fragmentation of nuclear DNA. This particular compound(s) which was present in the bacterial extract seemed to induce only a weak apoptotic signal that did not lead to complete progression of apoptosis.
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Holt JG, Krieg NR, Sneath PHA and Staley JT. 1994. Bergey’s Manual of Determinative Bacteriology. Nineteenth edition, Williams and wilkins company, Baltimore, MD, USA 255-273.
Jeong SY, Park SY, Kim YH, Kim M and Lee SJ. 2008. Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. J. of Appl. Microbio., 104:796-807.
Kerr JF, Wyllie AH and Currie AR. 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239-57.
Mizuki E, Park YS, Saitoh H, Yamashit S, Akao T, Higuchi K and Ohba M. 2000. Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis. Clin Diagn Lab Immunol. 7:625-634.
Mori H, Niwa K and Zheng Q. 2001. Cell proliferation in cancer prevention; effects of preventive agents on estrogen-related endometrial carcinogenesis model and on an in vitro model in human colorectal cells. Mutat Res, 480-1, 201-7.
Waksman SA and Woodruff HB. 1941. Actinomyces antibioticus, a new soil organism antagonistic to pathogenic and non-pathogenic bacteria. J. Bacteriol. 42: 231-249.
Weiss RB. 1992. The anthracyclines: will we ever find a better doxorubicin? Semin Oncol. 19:670-686.
Published
2012-07-25
How to Cite
Dan, V. M., Richard, V., Nair, A., Pushpangadan, P., George, V., Varma, A., & Pillai, R. (2012). Effect of extract of Bacillus megaterium 9554 on oral cancer cell line SCC131. Journal of Research in Biology, 2(5), 523-528. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/244
Section
Articles
