Criteria for optimization of mass rearing of the parasitoid Cotesia flavipes in the laboratory

Ramalho DG; Viel SR; Vacari AM; De Bortoli SA; Lopes MM; Laurentis VL; Veiga ACP

Ramalho DG laboratory of Biology and Insect Rearing (LBRI), Department of Crop Protection, College of Agricultural and Veterinary Sciences, Sao Paulo State University, 14884-900 Jaboticabal, Sao Paulo, Brazil
Viel SR Louis Dreyfus Commodities (Sugarcane Mill), 14870-904 Jaboticabal, Sao Paulo, Brazil
Vacari AM laboratory of Biology and Insect Rearing (LBRI), Department of Crop Protection, College of Agricultural and Veterinary Sciences, Sao Paulo State University, 14884-900 Jaboticabal, Sao Paulo, Brazil
De Bortoli SA laboratory of Biology and Insect Rearing (LBRI), Department of Crop Protection, College of Agricultural and Veterinary Sciences, Sao Paulo State University, 14884-900 Jaboticabal, Sao Paulo, Brazil
Lopes MM College of Education Sao Luis, 14870-37, Jaboticabal, Sao Paulo, Brazil
Laurentis VL laboratory of Biology and Insect Rearing (LBRI), Department of Crop Protection, College of Agricultural and Veterinary Sciences, Sao Paulo State University, 14884-900 Jaboticabal, Sao Paulo, Brazil
Veiga ACP laboratory of Biology and Insect Rearing (LBRI), Department of Crop Protection, College of Agricultural and Veterinary Sciences, Sao Paulo State University, 14884-900 Jaboticabal, Sao Paulo, Brazil

Keywords: Quality control, larval parasitoid, mass rearing, biological control, sugarcane

Abstract

In this study, we aimed to evaluate criteria that serve to optimize the mass rearing of the parasitoid Cotesia flavipes in the laboratory. The following studies were conducted: (1) Optimal density of the third instar larvae of Diatraea saccharalis was parasitized using a rearing unit, (2) Parasitism capacity during the lifespan of C. flavipes was examined, (3) Pupae of C. flavipes were weighed, and (4) Adult C. flavipes were fed with different diets. We found that a density of five larvae of D. saccharalis parasitized using a rearing unit is most suitable for the mass production of C. flavipes. A female C. flavipes may parasitize three larvae of D. saccharalis for up to 4 h after copulation, generating quality individuals for mass rearing. It is impractical to mass market the parasitoid C. flavipes by weight, the correct is to count the number of masses to form the release unit. A diet of 2.5% honey and 5% sucrose is most suitable for adults during the mass rearing of C. flavipes.

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Criteria for optimization of mass rearing of the parasitoid Cotesia flavipes in the laboratory

Abstract

References

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