In vitro response of Human Pre-osteoclasts to low intensity Laser irradiation
Keywords:
Pre-osteoclasts, Low energy laser, Cytotoxicity, Cell cycle, Orthodontic movement
Abstract
In vitro and in vivo studies have demonstrated that low intensity laser irradiation stimulates growth and cell differentiation of precursor cells, promoting dental movement and alveolar bone remodeling. But the information about the effect of laser irradiation on human pre-osteoclasts is limited.
Objective: To evaluate the effects on the viability of the pre-osteoclasts and cell proliferation in cultures of human pre-osteoclasts, after irradiation with low intensity laser.
Method: PoieticsTM Human Osteoclast Precursors Cat No. 2T-110 Cambrex-Lonza Inc. were irradiated with low intensity laser (As-Ga-Al) of 832.79 nm wavelength. A cytoxicity test was performed using the Lactate dehydrogenase (LDH) technique, measuring absorbance, 6 and 24 h after the treatment, in the Stat Fax-2100 at a wavelength of 492 nm. The pre-osteoclast cell density was measured by the absorbance every 24 h for 6 days, using a microplate reader. (Cell proliferation with Tetrazolium salts: kit XTT, Roche).
Results: The average cytotoxicity at 24 h was twice the observed at 6 h (59% difference) in the experimental group treated with laser; Triton cytotoxicity in the positive control group was seven times higher at 24 h (86.3% difference). After 6 h the laser was 30 times less cytotoxic than Triton and after 24 h was 89 times less cytotoxic than Triton (96,6% difference for 6 h and 98.8% difference for 24 h). These time differences are statistically significant (p<0.001). In the cell proliferation test the differences between groups were not statistically significant during the six days follow-up. Both cultures presented the same biological response, according to the cell cycle under study.
Conclusions: Low level laser irradiation does not have a cytotoxic effect that affect the cell viability in normal human Pre-osteoclasts cells cultured In vitro.
References
Abello M, Valbuena D. 1996. Efecto del láser blando (ir) en la velocidad de alineación del tratamiento ortodóncico. Tesis de postgrado Fundación C.I.E.O Bogotá, Colombia.
Aihara N, Yamaguchi M, Kasai K. 2006. Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in Vitro. Lasers Med Sci., 21(1):24-33.
Boyle W, Simonet S, Lacey D. 2003. Osteoclast differentiation and activation. Nature 423:337-423.
Cardaropoli D, Gaveglio L. 2007. The influence of orthodontic movement on periodontal tissues levels. Semin Orthod., 13(4):234-245.
Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC, Yum LW. 2001. The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res., 4(1):3-14.
Cruz DR, Kohara EK, Ribeiro MS, Wetter NU. 2004. Effects of low-intensity laser therapy on the orthodontic movement velocity of human teeth: a preliminary study. Lasers in Surgery and Medicine 35(2):117-120.
Dolce C, Malone S, Wheeler T. 2002. Current concepts in the biology of orthodontic tooth movement. Semin Orthod., 8(1):6-12.
Dominguez A, Castro P, Morales M. 2009. Cellular effects related to the clinical uses of laser in orthodontics. Journal of Oral laser Applications 9(4):199-203.
Dominguez A, Castro P, Morales M. 2009. In vitro Study of the reaction of human osteoblasts to low-level laser irradiation. J Oral Laser Applications 9(1):21-28.
Domínguez A, Clarkson A, López R. 2008. An in vitro study of the reaction of periodontal and gingival fibroblasts to low- level laser irradiation: A pilot study. J Oral Laser Applications 8(4):235-244.
Dominguez A, Velasquez S. 2010. Acceleration effect of orthodontic movement by application of low-intensity laser. J Oral.Laser Applications 10:99-105.
Fujihara NA, Hiraki KR, Marques MM. 2006. Irradiation at 780nm increases proliferation rate of osteoblasts independentdly of dexamethasone presence. Lasers Surg Med., 38(4):332-336.
Fujita S, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K. 2008. Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL. Orthod Craniofac Res., 11(3):143-155.
Karu TI. 1988. Molecular mechanism of the therapeutic effect of low-intensity laser radiation. Lasers Life Sci., 2(1):53-74.
Kawasaki K, Shimizu N. 2000. Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med., 26(3):282-291.
Kreisler M, Christoffers AB, Willershausen B, d'Hoedt B. 2003. Effect of low-level GaAlAs laser irradiation on the proliferation of human periodontal ligament fibroblasts: an in vitro study. J Clin Peridontol., 30(4):353-8.
Krishnan V, Davidovitch Z. 2006. Cellular, molecular, and tissue-level reactions to orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics 129(4):469-e1-32.
Krishnan V, Davidovitch Z. 2009. On a Path to Unfolding the Biological Mechanisms of Orthodontic Tooth Movement. J Dent Res., 88(7):597-608.
Lopes A, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM. 2001. Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med., 29(2):179-184.
Marques M, Pereira A, Fujihara NA, Nogueira FN, Eduardo CP. 2004. Effect of low-power laser irradiation on protein synthesis and ultra structure of human gingival fibroblasts. Lasers Surg Med., 34(3):260-5.
Masella RS, Eister M. 2006. Current concepts in the biology of orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 129(4):458-68.
McCulloch CAG. 1995; Origins and function of cells essential for periodontal repair: the role of fibroblasts in tissue homeostasis. Oral Diseases 1(4):271-278.
Meikle MC. 2006. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod., 28 (3):221-240.
Mikan J, Oliveros W. 2007. Osteoclastogénesis y enfermedades Óseas. Revista Med., 15:261-270.
Norton L. 2000. Fundamental principles of the biology of tooth movement. Semin Orthod., 6(3):139-144.
Noxon SJ, King GJ, Gu G, Huang G. 2001. Osteoclast clearance from periodontal tissues during orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 120(5):466-476.
Ozawa Y, Shimizu N, Kariya G, Abiko Y. 1998. Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22(4):347-354.
Pereira AN. Eduardo Cde P, Matson E, Marques MM. 2002. Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med., 31(4):263-7.
Pires Oliveira DA, De Oliveira RF, Zangaro RA, Soares CP. 2008. Evaluation of low-level laser therapy of osteoblastic cells. Photomed Laser Surg., 26(4):401-404.
Pompermaier T, Coelho U, Repeke C, Silva J, Queiroz F, Garlet GP. 2008. Differential expression of osteoblast and osteoclast chemo attractants in compression and tension sides during orthodontic movement. Cytokine 42(3):330-335.
Roberts WE, Goodwin WC. Jr, Heiner SR. 1981. Cellular response to orthodontic force. Dent Clin North Am., 25(1):3-17.
Rody WJ Jr, King GJ, Gu G. 2001. Osteoclast recruitment to sites of compression in orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 120(5):477-489.
Roodman D. 1996. Advances in bone biology: The Osteoclast. Endocrine Reviews 17(4):308-332.
Saito S, Shimizu N. 1997. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111(5):525-532.
Seifi M, Shafeei HA, Daneshdoost S, Mir M. 2007. Effects of two types of low-level laser wave lengths (850 and 630 nm) on the orthodontic tooth movements in rabbits .Lasers in Medical Science 22(4):261-4.
Sousa MV, Scanavini MA, Sannomiya EK, Velasco LG, Angelieri F. 2011. Influence of low-level laser on the speed of orthodontic movement. Photomed Laser Surg., 29(3):191-6.
Sun X, Zhu X, Xu C, Ye N, Zhu H. 2001. Effects of low energy laser on tooth movement and remodeling of alveolar bone in rabbits. Hua Xi Kou Qiang 19(5):290-293.
Tsay TP, Chen MH, Oyen OJ. 1999. Osteoclast activation and recruitment after application of orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics 115(3):323-330.
Vinck EM. Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC. 2003. Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci., 18(2):95-9.
Xie R, Kuijpers-Jagtman AM, Maltha JC. 2008. Osteoclast differentiation during experimental tooth movement by a short-term force application: An immunohistochemical study in rats. Act Odontol Scand 66(5):314-320.
Yamaguchi M, Hayashi M, Fujita S, Yoshida T, Utsunomiya T, Yamamoto H, Kasai K. 2010. Low energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha (v) beta (3) integrin in rats. European Journal of Orthodontics 32(2):131-139.
Aihara N, Yamaguchi M, Kasai K. 2006. Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in Vitro. Lasers Med Sci., 21(1):24-33.
Boyle W, Simonet S, Lacey D. 2003. Osteoclast differentiation and activation. Nature 423:337-423.
Cardaropoli D, Gaveglio L. 2007. The influence of orthodontic movement on periodontal tissues levels. Semin Orthod., 13(4):234-245.
Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC, Yum LW. 2001. The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res., 4(1):3-14.
Cruz DR, Kohara EK, Ribeiro MS, Wetter NU. 2004. Effects of low-intensity laser therapy on the orthodontic movement velocity of human teeth: a preliminary study. Lasers in Surgery and Medicine 35(2):117-120.
Dolce C, Malone S, Wheeler T. 2002. Current concepts in the biology of orthodontic tooth movement. Semin Orthod., 8(1):6-12.
Dominguez A, Castro P, Morales M. 2009. Cellular effects related to the clinical uses of laser in orthodontics. Journal of Oral laser Applications 9(4):199-203.
Dominguez A, Castro P, Morales M. 2009. In vitro Study of the reaction of human osteoblasts to low-level laser irradiation. J Oral Laser Applications 9(1):21-28.
Domínguez A, Clarkson A, López R. 2008. An in vitro study of the reaction of periodontal and gingival fibroblasts to low- level laser irradiation: A pilot study. J Oral Laser Applications 8(4):235-244.
Dominguez A, Velasquez S. 2010. Acceleration effect of orthodontic movement by application of low-intensity laser. J Oral.Laser Applications 10:99-105.
Fujihara NA, Hiraki KR, Marques MM. 2006. Irradiation at 780nm increases proliferation rate of osteoblasts independentdly of dexamethasone presence. Lasers Surg Med., 38(4):332-336.
Fujita S, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K. 2008. Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL. Orthod Craniofac Res., 11(3):143-155.
Karu TI. 1988. Molecular mechanism of the therapeutic effect of low-intensity laser radiation. Lasers Life Sci., 2(1):53-74.
Kawasaki K, Shimizu N. 2000. Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med., 26(3):282-291.
Kreisler M, Christoffers AB, Willershausen B, d'Hoedt B. 2003. Effect of low-level GaAlAs laser irradiation on the proliferation of human periodontal ligament fibroblasts: an in vitro study. J Clin Peridontol., 30(4):353-8.
Krishnan V, Davidovitch Z. 2006. Cellular, molecular, and tissue-level reactions to orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics 129(4):469-e1-32.
Krishnan V, Davidovitch Z. 2009. On a Path to Unfolding the Biological Mechanisms of Orthodontic Tooth Movement. J Dent Res., 88(7):597-608.
Lopes A, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM. 2001. Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med., 29(2):179-184.
Marques M, Pereira A, Fujihara NA, Nogueira FN, Eduardo CP. 2004. Effect of low-power laser irradiation on protein synthesis and ultra structure of human gingival fibroblasts. Lasers Surg Med., 34(3):260-5.
Masella RS, Eister M. 2006. Current concepts in the biology of orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 129(4):458-68.
McCulloch CAG. 1995; Origins and function of cells essential for periodontal repair: the role of fibroblasts in tissue homeostasis. Oral Diseases 1(4):271-278.
Meikle MC. 2006. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod., 28 (3):221-240.
Mikan J, Oliveros W. 2007. Osteoclastogénesis y enfermedades Óseas. Revista Med., 15:261-270.
Norton L. 2000. Fundamental principles of the biology of tooth movement. Semin Orthod., 6(3):139-144.
Noxon SJ, King GJ, Gu G, Huang G. 2001. Osteoclast clearance from periodontal tissues during orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 120(5):466-476.
Ozawa Y, Shimizu N, Kariya G, Abiko Y. 1998. Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22(4):347-354.
Pereira AN. Eduardo Cde P, Matson E, Marques MM. 2002. Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med., 31(4):263-7.
Pires Oliveira DA, De Oliveira RF, Zangaro RA, Soares CP. 2008. Evaluation of low-level laser therapy of osteoblastic cells. Photomed Laser Surg., 26(4):401-404.
Pompermaier T, Coelho U, Repeke C, Silva J, Queiroz F, Garlet GP. 2008. Differential expression of osteoblast and osteoclast chemo attractants in compression and tension sides during orthodontic movement. Cytokine 42(3):330-335.
Roberts WE, Goodwin WC. Jr, Heiner SR. 1981. Cellular response to orthodontic force. Dent Clin North Am., 25(1):3-17.
Rody WJ Jr, King GJ, Gu G. 2001. Osteoclast recruitment to sites of compression in orthodontic tooth movement. Am J Orthod Dentofacial Orthop., 120(5):477-489.
Roodman D. 1996. Advances in bone biology: The Osteoclast. Endocrine Reviews 17(4):308-332.
Saito S, Shimizu N. 1997. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111(5):525-532.
Seifi M, Shafeei HA, Daneshdoost S, Mir M. 2007. Effects of two types of low-level laser wave lengths (850 and 630 nm) on the orthodontic tooth movements in rabbits .Lasers in Medical Science 22(4):261-4.
Sousa MV, Scanavini MA, Sannomiya EK, Velasco LG, Angelieri F. 2011. Influence of low-level laser on the speed of orthodontic movement. Photomed Laser Surg., 29(3):191-6.
Sun X, Zhu X, Xu C, Ye N, Zhu H. 2001. Effects of low energy laser on tooth movement and remodeling of alveolar bone in rabbits. Hua Xi Kou Qiang 19(5):290-293.
Tsay TP, Chen MH, Oyen OJ. 1999. Osteoclast activation and recruitment after application of orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics 115(3):323-330.
Vinck EM. Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC. 2003. Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci., 18(2):95-9.
Xie R, Kuijpers-Jagtman AM, Maltha JC. 2008. Osteoclast differentiation during experimental tooth movement by a short-term force application: An immunohistochemical study in rats. Act Odontol Scand 66(5):314-320.
Yamaguchi M, Hayashi M, Fujita S, Yoshida T, Utsunomiya T, Yamamoto H, Kasai K. 2010. Low energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha (v) beta (3) integrin in rats. European Journal of Orthodontics 32(2):131-139.
Published
2012-12-24
How to Cite
Domínguez, A., Bayona, G., & Casas, A. (2012). In vitro response of Human Pre-osteoclasts to low intensity Laser irradiation. Journal of Research in Biology, 2(8), 733-741. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/275
Section
Articles
