Characterization of plumbagin from Plumbgo zeylanica L and screening of its impact on human intestinal micro flora
Abstract
The present study was carried out to investigate the antibacterial activity of plumbagin from Plumbago zeylanica against various human intestinal micro flora including Helicobacter pylori. Plumbagin, a bioactive compound was isolated from the root bark of P. zeylanica by fractional method using soxhlet apparatus, column chromatography and Thin Layer chromatography (TLC). The purity of the compound was further analyzed by subjecting the compound to HPLC studies. The minimum inhibitory concentration of active compound was tested against Staphylococcus aureus. Proteus vulgaris, Psedomanas aeruginosa, Escherichia coli and H. pylori. Almost all cases of peptic ulcers are caused by either H. pylori or the use of anti-inflammatory medication. The results showed that higher activity of plumbagin against human intestinal micro flora while compared to standard drugs. No growth was observed against probiotics, a friendly bacteria.
References
Daveis J. 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science, 264(5157): 375-382.
Desta B. 1993. Ethiopian traditional herbal drugs. Part II. Antimicrobial activity of 63 medicinal plants. Journal of Ethnopharmacology, 39(2): 129-139.
Durga R, Sridhar P and Polasa H. 1990. Effects of plumbagin, an antibiotic resistance in bacteria. Indian Journal of Medical Research, 91: 18-20.
Esquenazi D, Wigg MD, Miranda MM, Rodrigues HM, Tostes JB, Rozental S, da Silva AJ and Alviano CS. 2002. Antimicrobial and antiviral avtivities of Polyphenolics from cocos nucifera.Linn (Palmae) husk fiber extract. Research Microbiology, 153(10): 647-652.
Henry W. 2005. Helicobacter pylori and histopathological changes of gastric mucosa in Uganda population with varying prevalence of stomach cancer. African Health Science, 5(3): 234-237.
Hostettmannk, Wolfender JL and Rodriguez S. 1997.
Imlay J and Fridovich I. 1992. Exogenous quinones directly inhibit the respiratory NADH dehydrogenase in Escherichia coli. Archives of Biochemistry and Biophysics, 296(1): 337-346.
Karamani I, Sahin F, Gulluce M, Ogütçü H, Sengul M and Adiguzel A. 2003. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L,. Journal of Ethnopharmacology, 85(2-3): R31-5.
Mastroeni P. 2002. Immujnity to systemic salmonella infections. Current Molecular Medicine, 2(4): 393-406.
Nisha Mathew, Paily KP, Abidha, Vanamail P, Kalyanagundaram M and Balaraman K. 2002. Macrofilaricidal activity of the plant plumbago indica rosea in vitro. Drug Development Research, 56(1): 33-39.
Park, Byeoung and Soo-Seoul 2005. Selective growth inhibiting effects of compounds identified in Tabebuia Imputations a Inner bark on human intestinal bacteria. Journal of Agriculture and Food Chemistry, 53(4): 1152 – 1157.
Pietroiusti A, Luzzi I, Gomez MJ, Magrini A, Bergamaschi A, Forlini A and Galante A. 2005. Helicobacter pylori duodenal colonization is a strong risk factor for the development of duodenal ulcer. Alimentary Pharmacology and Therapeutics, 21(7): 909-915.
Hostettmann K, Wolfender JL and Rodriguez S. 1997. Rapid detection and subsequent isolation of bioactive constituents of crude plant extracts. Planta Medica, 63:(1) 2-10.
Selma, Riberio de Paiva. Maria RF, Tania V, Aragao MACK. 2003. Antimicrobial activity in vitro of plumbagin isolated from plumbago species. Mem Inst os valdo cruz, Ripde Janeiro, 98 (7): 959-961.
Van der Vijver LM 1972. Distribution of plumbagin in the plumbaginaceae. Phyotchemistry, 11(11): 3247-3248.
Villavicencio MA, Perez–Escandon BE 1992. Plumbagin activity from Plumbago pulchella Boiss. plumbnaginaceae) as a feeding deterrent for three speues of orthoptera. Folaia Entomol Mex 86 : 191-198.
Wang YC and Huang TL. 2005. High performance Liquid chromatography for quantificatin of plumbagin an anti Helicobacter pylori compound of Plumbago zeylanica. Journal of Chromatography, 1094(1-2): 99-104.
