Efficient in vitro micropropagation of Gynura procumbens - an important rare medicinal plant, through shoot tip and nodal segment explants

  • Farhana Parvin Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Jikrul Islam Md Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Naoshin Jahan Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Habiba Khan Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Pallob Ebna Shaekh Md Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Aminul Islam Md Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Muhammed Hamidur Rahaman Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
  • Motiur Rahman Md Assistant Professor, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205 Bangladesh
Keywords: Gynura procumbens, in vitro, micropropagation, cytokinin, auxin

Abstract

Gynura procumbens is a medicinally important herbaceous plant species belonging to the family Asteraceae. It works against virus, inflammation and various types of allergies. It is used to treat rheumatic fever, migraine, kidney disease, diabetes, dysentery, various types of skin diseases and cancers. This study aimed to develop a suitable protocol for rapid production of Gynura procumbens from different explants. Shoot tip and nodal segment explants were used from one year mature plant. For shoot proliferation, among the two explants, shoot tips showed the best response (90%) on Murashige and Skoog (MS) medium supplemented with 1.0mg/l BAP and produced an average of 20±0.8 shootlets in each explants. In vitro derived shoots were subcultured on the similar medium and it gave similar production with healthy shoots. 100% rooting was observed on full strength MS medium containing NAA (0.5mg/l). Rooted plantlets were transferred for hardening into the mixture of soil, cowdung and sand (1:1:1). Then the rooted plantlets were successfully established in the field.

References

Akowuah GA, Sadikun A, Mariam A, Aminah I. 2001. Blood sugar lowering Activity of Gynura procumbens leaf extracts. J. Trop. Med. Plants. 2(1): 5-10.

Agustina D, Haryana SM, Supartinah A. 2006. Anticarcinogenesis effect of Gynura procumbens (Lour) merr on tongue carcinogenesis in 4NQO-induced rat. Dental Journal (Maj. Ked. Gigi) 39(3):126- 132.

Bohari M, Pauliena S, Muhajir H, Khozirah S, Lajis N. 2006. Glucose uptake: Stimulatory activity of Gynura procumbens in 3T3-F442A adipocytes. In: Malaysian Medicinal plant: Chemistry and Biological Activity UNIMAS and Malaysian Natural Products Society, Sarawak.

Deka AC, Kalita MC, Baruah A. 1999. In Vitro Micropropagation of potent herbal medicinal plant, Withania somnifera (Ashwagandha). Environmental Ecology. 17(3): 594 – 596.

George S, Remashree AB, Sebastian D, Hariharan M. 2004. Micropropagation of Centella asiatica L. through axillary bud multiplication. Phytomorphology. 54: 31-34.

Iskander MN, Song Y, Coupar IM, Jiratchariyakul W. 2002. Antiinflammatory screening of the medicinal plant Gynura procumbens. Plant Food Hum Nutr. 57(3-4): 223-244.

Islam MM, Haque ME, Alam SMM, Islam MA, Khalekuzzaman M, Sikdar B. 2013. Morphological and Histological Observation of Embryogenic Calli Derived from Immature Embryo of BRRI Dhan28 (Oryza sativa L.) Variety. Res. in Plant Biol., 3(5): 21-27.

Islam MM, Roly ZY, Lee Y and Khalekuzzaman M. 2014. In vitro Propagation and Genetic Transformation System Using Immature Embryo in Elite Rice (Oryza sativa L.) Cultivars. The Korean Society of Breeding Science. 2(1): 88-96.

Jiratchariyakul W, Jarikasem S, Siritantikorn S, Somanabandhu A, Frahm AW. 2000. Antiherpes Simplex Viral Compounds from Gynura procumbens. Merr. (No.498) Mohidul University Annual Research Abstracts 28: 182.

Li WL, Ren BR, Zhuo M, Hu Y, Lu CG, Wu JL, Chen J, Shun S. 2009. The anti-hyperglycemic effect of plants in genus gynura cass. The American Journal of Chinese Medicine 37(5): 961- 966.

Mahmood AA, Mariod AA, Al-Bayaty F, Abdel-Wahab SI. 2010. Anti-ulcerogenic activity of Gynura procumbens leaf extract against experimentally-induced gastric lesionsin rats. Journal of Medicinal Plant Research 4(8): 685- 691.

Murashige T and Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiological Plantarum. 15(3):473-497.

Patnaik J and Debata BK. 1996. Micropropagation of Hemidesmus indicus (L.) R. Br. Through axillary bud culture. Plant Cell rep. 15(6): 427-430.

Puangpronpitag D, Chaichanadee S, Naowaratwattana W, Sittiwet C, Thammasarn K, Luerang A, Kaewseejan N. 2010. Evaluation of nutritional value and antioxidative properties of the medicinal plant Gynura procumbens extract. Asian Journal of Plant Sciences 9(3): 146- 151.

Raghu AV, Martin G, Priya V, Geetha SP, Balanchandran I. 2007. Low cost alternatives for the micropropagation of Centella asiatica J. Plant Sci. 2(6): 592-599.

Rosidah MFY, Sadikun A, Ahmad M, Akowuah GA, Asmawi MZ. 2009. Toxicology evaluation of standardized methanol extract of Gynura procumbens. Journal of Ethnopharmacology 123(2): 244- 249.

Sahoo Y, Pattnaik SK, Chand PK. 1997. In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (sweet basil) by axillary shoot proliferation. In Vitro Cellular and Developmental Biology - Plant 33(4):293–296.

Usha PK, Benjamin S, Mohanan KV, Raghu AV. 2007. An efficient micropropagation system for Vitex negundo L. an important woody aromatic medicinal plant, through shoot tip culture. Res J. Bot., 2(2): 102-107.
Published
2014-09-10
How to Cite
Parvin, F., Md, J. I., Jahan, N., Khan, H., Shaekh Md, P. E., Islam Md, A., Rahaman, M. H., & Rahman Md, M. (2014). Efficient in vitro micropropagation of Gynura procumbens - an important rare medicinal plant, through shoot tip and nodal segment explants. Journal of Research in Biology, 4(6), 1444-1450. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/362
Issue
Vol 4 No 6 (2014): Journal of Research in Biology (2014)
Section
Articles