Insecticide induced changes in haemolymph protein profiles of Spodoptera frugiperda (F) (Lepidoptera:Noctuidae)
Keywords:
Spodoptera frugiperda, insecticides, haemolymph proteins, induced changes
Abstract
Nine insecticides were evaluated for their toxicity (LC50) and 50% lethal times (LT50) against 3rd instar Spodoptera frugiperda larvae. Two groups of insecticides were identified based on LC50 and LT50 values. Bright® 30EC was the most toxic (LC50 = 0.0006 μg/g) while Fastac® 5EC was the least toxic (LC50 = 0.6046μg/g) among all the insecticides tested. Haemolymph protein changes from insecticide treated larvae were also determined. The total haemolymph protein content in insecticide treated larvae was generally lower than the control. Additionally, the number of protein bands present in electrophoresis gels of insecticide treated larvae was also lower than that of untreated larvae. The implications of these results are discussed.
References
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Carvalho EV, Gonclaves AH, Afférri FS, Dott MA and Peluzio JM. 2010. Influencia da lagarta-do-cartucho (Spodoptera frugiperda J.E. Smith), sobre hibridos de milho no sul do Tocantins-Brasil. Revista Verde de Agroecologia e Desenvolvimento Sustentável 5(5): 152-157.
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Tavares WS, Costa MA, Cruz I, Silveira RD, Serrao JE and Zanuncio JC. 2010. Selective effects of natural and synthetic insecticides on mortality of Spodoptera frugiperda (Lepidoptera:Noctuidae) and its predator Eriopis connexa (Coleoptera:Coccinellidae). Journal of Environmental Science and Health Part B 45(6): 557 – 561.
Usmani KA and Knowles CO. 2001. Toxicity of pyrethroids and effect of synergists to larval and adult Helicoverpa zea, Spodoptera frugiperda and Agrotis ipsilon (Lepidoptera:Noctuidae). Journal of Economic Entomology 94(4): 868-873.
Valdez-Lira JA, Alcocer-Gonzalez JM, Damas G, Nuñez-Mejía G, Oppert B, Rodriguez-Padilla C and Tamez-Guerra P. 2012. Comparative evaluation of phenoloxidase activity in different larval stages of four lepidopteran pests after exposure to Bacillus thuringiensis. Journal of Insect Science 12(80):1536-2442.
Yu JS. 1991. Insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith). Pesticide Biochemistry and Physiology 39(1): 84 – 91.
Ahmad M, Saleem MA and Ahmad M. 2005. Time oriented mortality in leafworm, Spodoptera litura (Fab.) (Lepidoptera:Noctuidae) by some new chemistry insecticides. Pakistan Entomologist 27(1): 67-70.
Carvalho EV, Gonclaves AH, Afférri FS, Dott MA and Peluzio JM. 2010. Influencia da lagarta-do-cartucho (Spodoptera frugiperda J.E. Smith), sobre hibridos de milho no sul do Tocantins-Brasil. Revista Verde de Agroecologia e Desenvolvimento Sustentável 5(5): 152-157.
Cruz I, Figueiredo MLC, Oliveira AC and Vasconcelos CA. 1999. Damage of Spodoptera frugiperda (Smith) in different maize genotypes cultivated in soil under three levels of aluminium saturation. International Journal of Pest Management 45(4): 293-296.
Labban O, Jugmohan H, Khan A, Matthew J and Wisdom S. 2012. Haemolymph composition of Ancylostomia stercorea Zeller (Lepidoptera:Pyralidae) larvae with particular reference to proteins and amino acids. Journal of Research in Biology 2(3): 178-183.
Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. 1951. Protein measurement with the Folin-Phenol reagent. Journal of Biological Chemistry 193(1): 265-275.
Mesnage R, Defarge N, de Vendômois J and Séralini GE. 2014. Major pesticides are more toxic to human cells than their declared active principles. BioMed Research International 2014 Article ID 179691. 8.
Nath BS, Suresh A, Varma BM and Kumar RPS. 1997. Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera:Bombycidae) in response to organophosphorus insecticides toxicity. Ecotoxicology and Environmental Safety 36(2): 169-173.
Sparks AN. 1979. A review of the biology of the fall armyworm. Florida Entomologist 62(2): 82-87.
Tavares WS, Costa MA, Cruz I, Silveira RD, Serrao JE and Zanuncio JC. 2010. Selective effects of natural and synthetic insecticides on mortality of Spodoptera frugiperda (Lepidoptera:Noctuidae) and its predator Eriopis connexa (Coleoptera:Coccinellidae). Journal of Environmental Science and Health Part B 45(6): 557 – 561.
Usmani KA and Knowles CO. 2001. Toxicity of pyrethroids and effect of synergists to larval and adult Helicoverpa zea, Spodoptera frugiperda and Agrotis ipsilon (Lepidoptera:Noctuidae). Journal of Economic Entomology 94(4): 868-873.
Valdez-Lira JA, Alcocer-Gonzalez JM, Damas G, Nuñez-Mejía G, Oppert B, Rodriguez-Padilla C and Tamez-Guerra P. 2012. Comparative evaluation of phenoloxidase activity in different larval stages of four lepidopteran pests after exposure to Bacillus thuringiensis. Journal of Insect Science 12(80):1536-2442.
Yu JS. 1991. Insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith). Pesticide Biochemistry and Physiology 39(1): 84 – 91.
Published
2014-11-12
How to Cite
Bart, Q., Indarsingh, J., Jugmohan, H., & Khan, A. (2014). Insecticide induced changes in haemolymph protein profiles of Spodoptera frugiperda (F) (Lepidoptera:Noctuidae). Journal of Research in Biology, 4(7), 1491-1497. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/368
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Articles
