Genetic Variation among Dwarf Gourami (Trichogaster lalia, Osphronemidae) populations using Random Amplified Polymorphic DNA(RAPD) markers
Keywords:
Genetic diversity, RAPD, Dwarf gourami
Abstract
Random amplified polymorphic DNA (RAPD) was applied to generate species-specific diagnostic fragment patterns for the molecular identification of the ornamental aquarium fish species Trichogaster lalia, more commonly known as dwarf gourami. The species were collected from various geographically distant locations of Assam. After initial screening, four primers having a length of 10 arbitary nucleotide sequence were used which generated the RAPD profile for Trichogaster species. The primers produced 39 bands in total. In the experiment 22 polymorphic bands and 7 monomorphic bands were produced. The genetic distance of an individual ranged from 0.03 to 0.38. The average genetic distance among the individuals showed that more than 0.03 species are genetically more similar
References
Ayoma J, Watanbe S, Nishida M and Tsukamato K 2000 Discrimination of catadroumous eels of genus Anguilla using polymerase chain reaction restriction fragment length polymorphism analysis of the mitochondrial DNA 16S ribosomal RNA domain. TransAm Fish Soc 129:873-878.
Brahmane MP, Das MK, Sinha MR, Sugunan VV, Mukherjee A, Singh SN, Prakash S, Maurye P and Hajra 2006. A.Use of RAPD fingerprinting for delineating populations of hilsa shad Tenualosa ilisha (Hamilton 1822). Genet Mol Res. 31:643-652.
Cadrin SX 2000. Advances in morphometric identification of fishery stocks. Reviews in Fish Biology and Fisheries. 10, 91–112.
Das P and Pandey AK 1998. Current status of fish germplasm resource of India and strategies for conservation of endangered species. In: Ponniah AG and Das P (eds) Fish Genetics and Biodiversity Conservation. Nature Conservators, Muzzaffarnagar pp 253-273.
Dinesh KR, Lim TM, Chan WK and Phang 1993 VPE RAPD analysis: An efficient method of DNA fingerprinting in fishes. Zool Sci 10:849-854.
Frankham 1995 “Conservation genetics,” Annual Review of Genetics, vol. 29 pp. 305–327,
Garg RK, Silawat N, Sairkar P, Vijay N and Mehrotra NN. 2009. RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India.Afric. J. Biotechnol., 8 (17)4032-4038.
Garg RK, Silawat N, Sairkar P, Vijay N and Mehrotra NN. 2009b. Genetic diversity between two populations of Heteropneustes fossilis (Bloch) using RAPD profile. Int. J. Zool. Res., 5: 171-177.
Hamilton F. 1822. An account of the fishes found in the river Ganges and its branches. Archibald Constable and Company, Edinburgh, 405 pp.
Hadrys H, Balick M and Schierwater B. 1992. “Applications of random amplified polymorphic DNA (RAPD) in molecular ecology,” Molecular Ecology, vol. 1, no. 1, pp. 55–63,
Ibanez-Aguirre AL, Cabral-Solis E, Gallardo-Cabello M, Espino-Barr E 2006. Comparative morphometrics of two populations of Mugil curema (Pisces: Mugilidae) on the Atlantic and Mexican Pacific coasts. Scientia Marina 70 (1) 139–45
Jayaram KC. 2002. The Fresh Water Fishes of the Indian Region. Narendra Publishing House, Delhi.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, and SV. 1990. Tingey, “DNA polymorphisms amplified by arbitrary primers are useful as genetic markers,” Nucleic Acids Researchvol. 18, no. 22, pp 6531–6535.
Lynch M and B. G. Milligan 1994, “Analysis of population genetic structure with RAPD markers,” Molecular Ecology, vol. 3, no. 2,pp. 91–99,
Nei M. 1978. “Estimation of average heterozygosity and genetic distances from small number of individuals. Genetics 89583-590.
Sambrook J and Russell DW. 2001 “Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, New York.
Sneath PHA and Sokal RR. 1973. Numerical taxonomy. Freeman, San Francisco.
Talwar PK and Jhingran AG. 1991. Inland Fishes of India and adjacent countries. Oxford and IBH, New Delhi, 1158
Welsh J and McClelland Mm. 1990, “Fingerprinting genomes using PCR with arbitrary primers,” Nucleic Acids Research, vol. 18, no. 24,pp7213.
Brahmane MP, Das MK, Sinha MR, Sugunan VV, Mukherjee A, Singh SN, Prakash S, Maurye P and Hajra 2006. A.Use of RAPD fingerprinting for delineating populations of hilsa shad Tenualosa ilisha (Hamilton 1822). Genet Mol Res. 31:643-652.
Cadrin SX 2000. Advances in morphometric identification of fishery stocks. Reviews in Fish Biology and Fisheries. 10, 91–112.
Das P and Pandey AK 1998. Current status of fish germplasm resource of India and strategies for conservation of endangered species. In: Ponniah AG and Das P (eds) Fish Genetics and Biodiversity Conservation. Nature Conservators, Muzzaffarnagar pp 253-273.
Dinesh KR, Lim TM, Chan WK and Phang 1993 VPE RAPD analysis: An efficient method of DNA fingerprinting in fishes. Zool Sci 10:849-854.
Frankham 1995 “Conservation genetics,” Annual Review of Genetics, vol. 29 pp. 305–327,
Garg RK, Silawat N, Sairkar P, Vijay N and Mehrotra NN. 2009. RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India.Afric. J. Biotechnol., 8 (17)4032-4038.
Garg RK, Silawat N, Sairkar P, Vijay N and Mehrotra NN. 2009b. Genetic diversity between two populations of Heteropneustes fossilis (Bloch) using RAPD profile. Int. J. Zool. Res., 5: 171-177.
Hamilton F. 1822. An account of the fishes found in the river Ganges and its branches. Archibald Constable and Company, Edinburgh, 405 pp.
Hadrys H, Balick M and Schierwater B. 1992. “Applications of random amplified polymorphic DNA (RAPD) in molecular ecology,” Molecular Ecology, vol. 1, no. 1, pp. 55–63,
Ibanez-Aguirre AL, Cabral-Solis E, Gallardo-Cabello M, Espino-Barr E 2006. Comparative morphometrics of two populations of Mugil curema (Pisces: Mugilidae) on the Atlantic and Mexican Pacific coasts. Scientia Marina 70 (1) 139–45
Jayaram KC. 2002. The Fresh Water Fishes of the Indian Region. Narendra Publishing House, Delhi.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, and SV. 1990. Tingey, “DNA polymorphisms amplified by arbitrary primers are useful as genetic markers,” Nucleic Acids Researchvol. 18, no. 22, pp 6531–6535.
Lynch M and B. G. Milligan 1994, “Analysis of population genetic structure with RAPD markers,” Molecular Ecology, vol. 3, no. 2,pp. 91–99,
Nei M. 1978. “Estimation of average heterozygosity and genetic distances from small number of individuals. Genetics 89583-590.
Sambrook J and Russell DW. 2001 “Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, New York.
Sneath PHA and Sokal RR. 1973. Numerical taxonomy. Freeman, San Francisco.
Talwar PK and Jhingran AG. 1991. Inland Fishes of India and adjacent countries. Oxford and IBH, New Delhi, 1158
Welsh J and McClelland Mm. 1990, “Fingerprinting genomes using PCR with arbitrary primers,” Nucleic Acids Research, vol. 18, no. 24,pp7213.
Published
2015-05-16
How to Cite
Das, M., & MM, G. (2015). Genetic Variation among Dwarf Gourami (Trichogaster lalia, Osphronemidae) populations using Random Amplified Polymorphic DNA(RAPD) markers. Journal of Research in Biology, 5(3), 1713-1719. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/381
Section
Articles
