Activity of alpha amylase in the tracheobronchial secretions of patients without morbid salivary aspiration
Abstract
Background
The normal values of α-amylase in humans is unknown.
Objective
To determine the normal values of α-amylase activity in humans.
Material and methods
From October 2009 to June 2011 we studied 107 patients referenced to thoracic service to be submitted to bronchoscopy.
The patients were positioned in supine position, performed local antisepsis and anesthetized with 2% lidocaine. Thereafter we introduced a needle into catheter by puncturing the cricothyroid membrane using a 14G cateter. Finally we introduced ten milliliters of saline and immediately aspirated with the maximum power of the vacuum system. The samples were sent for α- amylase activity determination by CNPG method.
Results
The activity of α-amylase of tracheal aspirate ranged from 24 to 10.000 IU/l), and a mean 1914IU/L. The levels had no statistical differences according age, sex, race, smoking history and the lung diseases.
Conclusion
We could define the probably physiologic levels of α-amylase activity in humans beings.
References
Amberson JB Jr. (1937). Aspiration bronchopneumonia. International Anesthesiology Clinics, 3(6): 126
Clarke PD, Bain BC, Davies A, Levin GE and Lambert HP. (1981). Aspiration in seriously ill patients: a study of amylase in bronchial secretions. Journal of Clinical Pathology, 34(7): 803-805.
Gleeson K, Eggli DF and Maxwell SL. (1997). Quantitative aspiration during sleep in normal subjects. Chest Journal, 111(5): 1266-72.
Huxley EJ, Viroslav J, Gray WR and Pierce AK. (1978). Pharyngeal aspiration in normal adults and patients with depressed consciousness. The American Journal of Medicine, 64(4): 564-568.
Logemann JA, Rademaker AW, Pauloski BR, Ohmae Y and Kahrilas PJ. (1998). Normal swallowing physiology as viewed by video fluoroscopy and video endoscopy. Folia Phoniatr Logop, 50(6): 311-319.
Morishita Y, Iinuma Y, Nakashima N, Majima K, Mizuguchi K and Kawamura Y. (2000). Total and pancreatic amylase measured with 2-chloro-4-nitrophenyl-4-O-beta-D-galactopyranosylmaltoside. Clinical Chemistry. 46(7): 928-933.
Nandapalan V, McIlwain JC and England J. (1995a). Amylase activity in tracheobronchial secretions of laryngectomized patients. The Journal of Laryngology and Otology, 109(7): 637-639.
Nandapalan V, McIlwain JC and Hamilton J. (1995b). A study of alpha-amylase activity in tracheobronchial secretions of seriously ill patients with tracheostomies. The Journal of Laryngology and Otology. 109(7): 640-643.
Pecora DV. (1959). A method of securing uncontaminated tracheal secretions for bacterial examination. The Journal of Thoracic Surgery, 37(5): 653-4.
Pecora DV. (1970). Transtracheal aspiration. American family physician GP, 1(3): 99.
Pratter MR and Irwin RS. (1979). Transtracheal aspiration. Guidelines for safety. Chest Journal, 76(5):518-20, 1970 1(3): 99.
Sano M, Zennami S and Masaoka A. (1986). Activities of some enzymes in tracheal and bronchial fluid. Panminerva Medica. 28(2): 137-142.
