In vitro and in vivo potentiation of ampotericin-B by flavonoid against different fungal strains
Abstract
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavoniods with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. The flavonoid, flavones and diglycoside (3, 5-dihydroxy flavones, 7-O-b-D-galacturonids-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of Ampotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion these finding suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
References
Betoni JEC, Mantovani RP, Barbosa LN, Di Stasi LC and Fernandes A Jnr. (2006). Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Memórias do Instituto Oswaldo Cruz, 101(4): 387-390.
Cushnie TPT and Lamb AJ. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5): 343-356.
Harborne JB and Williams CA. (2000). Advance in flavonoid research since 1992. Phytochemistry, 55(6): 481-504.
Harvey AL. (1999). Medicines from nature: are natural products still relevant for drug discovery? Trends in pharmacological sciences, 20(5): 196-198.
National Committee for Clinical Laboratory Standards. (2000). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th ed. Approved standard. NCCLS publication no. M07-A5. National Committee for Clinical Laboratory Standards, Wayne, PA, 19087 USA.
Nascimento GGF, Locatelli J, Freitas PC and Silva GL. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4): 247-256.
Nahrstedt A. (1997). Natural products from higher plants as lead for drug development. European Journal of Pharmaceutical Sciences, 5(suppl): S18.
Okemo PO, Mwatha WE, Chhabrab SC and Fabryc W. (2001). The kills kinetics of Azadirachta indica A. JUSS. (MELIACEAE) Extracts on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. African Journal of Science and Technology, 2(2): 113-118
Oluwatuyi M, Kaatz GW and Gibbons S. (2004). Antibacterial and resistance modifying activity of Rosmarinus officinalis. Phytochemistry, 65(24): 3249-3254.
Pankey G, Ashcraft D and Patel N. (2005). In vitro Synergy of Daptomycin plus Rifampin agains-Enterococcus faecium Resistant to both Linezolid and Vancomycin. Antimicrobial Agents Chemotherapy, 49(12): 5166- 5168.
Patel M and Coogan MM. (2008). Antifungal activity of the plant Dodonaea viscosa var. angustifolia on Candida albicans from HIV-infected patients. Journal of Ethnopharmacology, 118(1): 173-176.
Reed LJ and Muench H. (1938). A simple method of estimating fifty percent endpoints. The American Journal of Hygiene, 27(3): 493-497.
Rabe T and van Staden J. (2000). Isolation of an antibacterial sesquiterpenoid from Warburgia salutaris. Journal of Ethnopharmacology, 73(1-2):171-4
Rukayadi Y, Yong D and Hwang JK. (2006). In vitro anticandidal activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. Journal of Antimicrobial Chemotherapy, 57(6): 1231-1234.
Prescott LM, Harley JP and Klein DA. (2002). Microbiology 6th ed. Macgraw Hill Publishers, 823 p.
Sibanda T and Okoh AI. (2008). In vitro evaluation of the interactions between acetone extracts of Garcinia kola seeds and some antibiotics. African Journal of Biotechnology, 7(11): 1672-1678.
Sato Y, Shibata H, Arai T, Yamamoto A, Okimura Y, Arakaki N and Higuti T. (2004). Variation in synergistic activity by flavone and its related compounds on the increased susceptibility of various strains of methicillin-resistant Staphylococcus aureus to β-lactam antibiotics. International Journal of Antimicrobial Agents, 24(3): 226-233.
