In vitro multiplication of the endangered plant species, Exacum bicolor Roxb
Keywords:
Micropropagation, Exacum bicolor, Shoot proliferation, in vitro shoots
Abstract
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
References
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Jeeshna Meethaley Valappil and Paulsamy Subramaniam. (2011). Evaluation of certain flavonoids of medicinal importance in the wild and micropropagated plants of the endangered medicinal species, Exacum bicolor Roxb. Journal of Applied Pharmaceutical Science, 1(5): 99-102
Joshi P and Dhawan V. (2007). Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR maker assay boil plant. Biologia Plantarum, 51(1): 22-26.
Kannan P, Premkumar A and Ignacimuthu S. (2007). Thidiazuron induced shoot regeneration in the endangered species, Exacum travancoricum Beedi. Indan Journal of Biotechnolgy, 6(4): 564 – 566.
Karuppusamy S and Pullaiah T. (2007). In vitro shoot multiplication of Bupleurum distichophyllum Wight – A native medicinal plant of Southern India. Plant Tissue Culture and Biotechnology, 17(2): 115-124.
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Kurt S and Erdag B. (2009). In vitro germination and axillary shoot propagation of Centaurea zeybek. Biologia, 64(1): 97-101.
Mallikadevi T, Senthilkumar P and Paulsamy S. (2008). In vitro regeneration of the medicinal plant, Plumbago zeylanica L. with reference to a unique population in Maruthamalai, the Western Ghats, India. Plant Tissue Culture and Biotechnology, 18(2): 173-179.
Nanda R, Das P and Rout G. (2004). In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annuals of Forest Science, 61(4): 381-386.
Nasirujjaman K, Salah Uddin M, Zaman S and Reza, MA. (2005). Micropopagation of turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture. Journal of Biological Sciences, 5(4): 490-492.
Ornstrup H, Molgaard JP and Farestveit B. (1993). Somatic embryo-genesis and plant regeneration from cell suspensions of Exacum affine. Plant Cell Tissue and Organ Culture, 35(1): 37-40.
Padmavathy K, Paulsamy S and Thambiraj J. (2013). In vitro shoot regeneration of Corchorus acutangulus Lam. (Tiliaceae). Research and Reviews: Journal of Botanical Sciences, 2(3): 1-5.
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Sahoo Y and Chand PK. (1998). Micropropagation of Vitex negundo L, a woody aromatic medicinal shrub, through high – frequency axillary shoot proliferation. Plant cell Reports, 18(3): 301 – 307.
Sarwar S, Zia M, Rehman R, Fatima Z, Sial RA and Chaudhary MF. (2009). In vitro direct regeneration in mint from different explants on half strength MS medium. African Journal of Biotechnology 8(18): 4667-4671.
Senthilkumar P, Paulsamy S and Mallikadevi T. (2009). In vitro regeneration of an important medicinal plant, Ophiorrhiza mungos L. Journal of Agriculture, Biotechnology and Ecology, 2(3): 322-330.
Shiddamallayya N, Yesmeen A and Gopakumar K. (2010). Medico – botanical survey of Kumar Parvatha Kukke Subramanya, Mangalore. Indian Journal of Traditional Knowledge, 9(1): 96 – 99.
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Vengadesan G, Ganapathi A and Anand RP Anbazhagan VR. (2002). In vitro propagation of Acacia sinuata (Lour.) Merr. Via cotyledonary nodes. Agroforestry Systems, 55(1): 9-15.
Vengadesan G, Ganapathi A, Anand RP and Selvaraj N. (2003). In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of 10 - years old-tree. In Vitro Cellular and Developmental Biology - Plant, 39(4): 409-414.
Vinterhalter B, Jankovic T, Savikin K, Nikolic R and Vinterhalter D. (2008). Propagation and xanthone content of Gentianella austriaca shoot cultures. Plant Cell Tissue and Organ Culture, 94: 329-335.
Beck SL and Dunlop RW. (2001). Micropropagation of the Acacia species- A review. In vitro Cellular and Developmental Biology-Plant, 37(5): 531-538.
Dewan A, Nanda K and Gupta SC. (1992). In vitro micropropagation of Acacia nilotica sub sp. Indica Brenan. Via cotyledonary nodes. Plant cell Reports, 12(1): 18-21.
Gomez KA and Gomez KA. (1976). Statistical procedure for agricultural research with emphasis of Rice. Los Bans. Philipines International Rice Research Institute Book. Los Banos, Philipines. A Wiley-intersclence Publication.
Jeeshna MV. (2011). Evaluation of ecological variants of the endangered plant species, Exacum bicolor Roxb.(Gentianaceae) for effective medicinal usage and in vitro regeneration protocol for its conservation. (Dissertation). Bharathiyar University. Coimbatore, Tmil Nadu, India.
Jeeshna Meethaley Valappil and Paulsamy Subramaniam. (2011). Evaluation of certain flavonoids of medicinal importance in the wild and micropropagated plants of the endangered medicinal species, Exacum bicolor Roxb. Journal of Applied Pharmaceutical Science, 1(5): 99-102
Joshi P and Dhawan V. (2007). Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR maker assay boil plant. Biologia Plantarum, 51(1): 22-26.
Kannan P, Premkumar A and Ignacimuthu S. (2007). Thidiazuron induced shoot regeneration in the endangered species, Exacum travancoricum Beedi. Indan Journal of Biotechnolgy, 6(4): 564 – 566.
Karuppusamy S and Pullaiah T. (2007). In vitro shoot multiplication of Bupleurum distichophyllum Wight – A native medicinal plant of Southern India. Plant Tissue Culture and Biotechnology, 17(2): 115-124.
Khare CP. (2007). Indian Medicinal Plants an illustrated dictionary. Springer Science, Business Media, LLC, New York.
Kurt S and Erdag B. (2009). In vitro germination and axillary shoot propagation of Centaurea zeybek. Biologia, 64(1): 97-101.
Mallikadevi T, Senthilkumar P and Paulsamy S. (2008). In vitro regeneration of the medicinal plant, Plumbago zeylanica L. with reference to a unique population in Maruthamalai, the Western Ghats, India. Plant Tissue Culture and Biotechnology, 18(2): 173-179.
Nanda R, Das P and Rout G. (2004). In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annuals of Forest Science, 61(4): 381-386.
Nasirujjaman K, Salah Uddin M, Zaman S and Reza, MA. (2005). Micropopagation of turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture. Journal of Biological Sciences, 5(4): 490-492.
Ornstrup H, Molgaard JP and Farestveit B. (1993). Somatic embryo-genesis and plant regeneration from cell suspensions of Exacum affine. Plant Cell Tissue and Organ Culture, 35(1): 37-40.
Padmavathy K, Paulsamy S and Thambiraj J. (2013). In vitro shoot regeneration of Corchorus acutangulus Lam. (Tiliaceae). Research and Reviews: Journal of Botanical Sciences, 2(3): 1-5.
Pande D, Srivastava PS and Ramasamy NS. (2000). Xanthotoxin in tissue cultures of Ammi majus Linn. Journal of Tropical Medicinal Plants, 1(1-2): 43-52.
Pullaiah T. (2006). Encyclopaedia of World medicinal plant. Volume 2, Regency pub., West Patel Nagar, New Delhi.
Purohit SDA, Dave A and Kukda G. (1994). In vitro conservation strategies for a rare medicinal herb–safed muslii (Chlorophytum borivilianum Sant. Etfernand). Indian Journal of Plant Genetic Resources, 7(2): 201 – 204.
Reddi STV, Naidu BVAR and Prasanthi S. (2005). In : Herbal remedies for diseases. I Alikhan and AKhanum (Eds), Ukay Publications, Hyderabad.
Rout GR, Senapati SK and Aparajetha S. (2008). Micropropagation of Acacia chundra (Roxb.) DC. Hort Science. 35(1): 22-26.
Sahoo Y and Chand PK. (1998). Micropropagation of Vitex negundo L, a woody aromatic medicinal shrub, through high – frequency axillary shoot proliferation. Plant cell Reports, 18(3): 301 – 307.
Sarwar S, Zia M, Rehman R, Fatima Z, Sial RA and Chaudhary MF. (2009). In vitro direct regeneration in mint from different explants on half strength MS medium. African Journal of Biotechnology 8(18): 4667-4671.
Senthilkumar P, Paulsamy S and Mallikadevi T. (2009). In vitro regeneration of an important medicinal plant, Ophiorrhiza mungos L. Journal of Agriculture, Biotechnology and Ecology, 2(3): 322-330.
Shiddamallayya N, Yesmeen A and Gopakumar K. (2010). Medico – botanical survey of Kumar Parvatha Kukke Subramanya, Mangalore. Indian Journal of Traditional Knowledge, 9(1): 96 – 99.
Sreelatha U, Baburaj TS and Narayan Kutty C. (2007). Exacum bicolor- A elegant wild flowering herbs. Underutilized and Underexploited horticultural crops. New India Publishing Agency, New Delhi.
Vengadesan G, Ganapathi A and Anand RP Anbazhagan VR. (2002). In vitro propagation of Acacia sinuata (Lour.) Merr. Via cotyledonary nodes. Agroforestry Systems, 55(1): 9-15.
Vengadesan G, Ganapathi A, Anand RP and Selvaraj N. (2003). In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of 10 - years old-tree. In Vitro Cellular and Developmental Biology - Plant, 39(4): 409-414.
Vinterhalter B, Jankovic T, Savikin K, Nikolic R and Vinterhalter D. (2008). Propagation and xanthone content of Gentianella austriaca shoot cultures. Plant Cell Tissue and Organ Culture, 94: 329-335.
Published
2016-07-04
How to Cite
MV, J. (2016). In vitro multiplication of the endangered plant species, Exacum bicolor Roxb. Journal of Research in Biology, 6(5), 2054-2061. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/422
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