Evaluation of anti-bacterial potential of protein isolated from the muscle of Channa striatus
Abstract
Protein was extracted from muscles of Channa striatus and attempts were made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp (19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16 mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5 mm). The minimum inhibitory concentration and minimum bactericidal concentration were found to be 20-40 μg/ml and 80-100 μg/ml respectively for the extracts of Channa striatus protein against test organisms. This study confirms that C. striatus fish protein extracts possess antibacterial activity against a wide range of microbes and justified that it could be used in the traditional medicine as a remedy for the treatment of bacterial diseases.
References
Frieden TR, Sterling TR, Munsiff SS and Watt CJ. (2003). Tuberculosis. Lancet, 362: 887-899.
Garg SC and Jain RK. (1998). Antimicrobial efficacy of essential oil found Curcuma caesia. Indian Journal of Microbiology, 38(3): 169–170.
Grange JM. (1998). Tuberculosis In Hausler W. Jrsussman M. eds. Topleyand Willsons Microbiology and Microbial infections. Oxford University Press, New York.
Ilavazhahan M, Tamil Selvi R and Jayaraj SS. (2010). Determination of LC50 of the bacterial pathogen, pesticide and heavy metal for the fingerling of freshwater fish Catla catla. Global Journal of Environmental Research, 4(2): 76-82.
Irobi ON, Moo-Young M and Anderson WA. (1996). Antimicrobial activity of Annatto (Bixa orellana) extract. International Journal of Pharmacology, 34: 87–90.
Lighty George, Padmalatha C, Ranjitsingh AJA and Dhasarathan P. (2010). Antibacterial potential of haemolymph and aqueous extraction of red velvet mite, T. grandissimum. Journal of Biomedical Sciences and Research, 2(4): 250-253.
Paulsi S and Dhasarathan P. (2011). Estimation of biochemical status and immunostimulant potential of chosen fruits. International Journal of Pharma and Biosciences, 2(1): 90-97.
Samy PR and Ignacimuthu M. (2001). Antibacterial extracts of the bark of Terminalia arjuna justification of folklare belief. Pharamceutical Biology, 39(6): 417-420.
Sujatha M, Dhasarathan P and Karthick B. (2010). Antibiotic susceptibility and immunomodulatory potential of chosen bacterial pathogens, American Journal of Immunology, 6(2): 15-19.
Tekle YI, Nielsen KM, Liu J, Pettigrew MM, Meyers LA, Galvani AP and Townsend JP. (2012). Controlling antimicrobial resistance through targeted vaccine –induced replacement of strains. PLoS ONE, 7(12): e50688.
Thorarinsson R and Powell DB. (2006). Effects of disease risk, vaccine efficacy and market price on the economics of fish vaccination. Aquaculture, 256: 42-49.
Zhang CL, Pearson A, Li Y-L, Mills G and Wiegel J. (2006). A thermophilic temperature optimum for crenarchaeol and its implication for archaeal evolution. Applied and Environmental Microbiology, 72: 4419-4422.
