Comparative evaluation of hyaluronic acid production by Streptococcus thermophilus isolated from yoghurt
Keywords:
Hyaluronic acid, Streptococcus thermophilus, FTIR
Abstract
Hyaluronic acid (HA) is also known by the name hyaluronan. The necessity for using this fabulous material lead to investigate non-pathogenic strains which produce this material. The most non-pathogenic strain is S. thermophilus. The lack of literature on microbial production of this substance by the strain prompted us to examine the microbial production of HA from it and also to examine optimization of culture conditions where HA is produced. The bacteria Streptococcus salivarius sub. thermophilus was obtained from the Bank of Scientific and Industrial Research of Iran (PTCC 1738). To separate S. thermophilus strains from yogurts, three types of yogurts were used. They were cultured by pour-plate and surface methods on STA medium. To identify the isolated strains, biochemical tests and Polymerase Chain Reaction (PCR) were used. Bacterial strains isolated from yoghurts were identified as S. thermophilus MN-BM-A02, S. thermophilus JIM8232 and S. thermophilus MN-ZLW-002. To separate the capsule strains, each strain was cultured on STB medium and then they were centrifuged. In order to purify the samples, ethanol and charcoal were used. To optimize production, variety of sources of carbon, nitrogen, temperature and pH were studied.
References
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Weisburg WG, Barns SM, Pelletier DA and Lane DJ. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173(2): 697-703.
Wang J, Zhao X, Tian Z, Yang Y and Yang Z. (2015). Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir. Carbohydrate Polymers, 125: 16–25.
Zhang Q, Ren J, Zhao H, Zhao M, Xu J and Zhao Q. (2011). Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. International Journal of Food Science and Technology, 46(5): 1014-1020.
Boeriu CG, Springer J, Kooy FK, Broek VDL and Eggink G. (2013). Production methods for hyaluronan, International Journal of Carbohydrate Chemistry, 94(2): 295–305.
Botelho PS, Maciel MI, Bueno LA, Maria de Fatima FM, Marques DN and Silva TMS. (2014). Characterisation of a new exopolysaccharide obtained from of fermented kefir grains in soymilk. Carbohydrate Polymers, 107: 1-6.
Cappuccino James and Natalie S. (2004). Microbiology a laboratory manual. 7th ed. Benjamin Cummings. 544 p.
Degeest B and De Vuyst L. (1999). Indication that the nitrogen source influences both amount and size of exopolysaccharides produced by Streptococcus thermophilus LY03 and modeling of the bacterial growth and exopolysaccharide production in a complex medium. Applied and Environmental Microbiology, 65(7): 2863-2870.
Donghui F, Beibei W, Zheng X and Qisheng G. (2006). Determination of hyaluronan by spectroscopic methods. Journal of Wuhan University of Technology-Mater. Sci. Ed., 21(3): 32-34.
Dyk JSV, Kee NLA, Frost CA and Pletschke BI. (2012). Extracellular polysaccharide production in Bacillus licheniformis SVD1 and its immunomodulatory effect. Bio Resources, 7(4): 976–4993.
Huggins ML. (1942). The viscosity of dilute solutions of long-chain molecules. IV. Dependence on concentration. Journal of the American Chemical Society, 64(11): 2716-2718.
Im JH, Song JM, Kang JH and Kang DJ. (2009). Optimization of medium components for high-molecular-weight hyaluronic acid production by Streptococcus sp. ID9102 via a statistical approach. Journal of Industrial Microbiology and Biotechnology, 36(11): 1337-1344.
Ismail B and Nampoothiri KM. (2010). Production, purification and structural characterization of an exopolysaccharide produced by a probiotic Lactobacillus plantarum MTCC 9510. Archives of Microbiology, 192(12): 1049–1057.
Kaushik K, Kumar N and Pathak D. (2012). Synthesis of some newer carbazole derivatives and evaluation for their pharmacological activity. Der Pharmacia Sinica, 3(4): 470-474.
Kirsop BE and Doyle A. (1991). Maintenance of microorganisms and cultured cells. 2nd ed. 17-18 p.
Kogan G, Soltes L, Stern R and Mendichi R. (2007). Hyaluronic acid: a biopolymer with versatile physico-chemical and biological properties-Handbook of Polymer Research. Nova Science and Publishers, 393-439 p.
Kumar MA, Anandapandian KTK and Parthiban K. (2011). Production and characterization of Exopolysaccharides (EPS) from biofilm forming marine bacterium. Brazilian Archives of Biology and Technology, 54(2): 259–265.
Liu M. (2011). Sterile hyaluronic acid solutions, WO2011100114 A1,US Patent.
Murray HD, Schneider DA and Gourse RL. (2003). Control of rRNA expression by small molecules is dynamic and nonredundant. Molecular Cell, 12(1): 125-134.
Naoki I and Tomoko H. (2008). Streptococcus thermophilus produces exopolysaccharides including hyaluronic acid. Journal of Bioscience and Bioengineering, 107(2): 119-123.
Necas J, Bartosikova L, Brauner P and Kolar J. (2008). Hyaluronic acid (hyaluronan): a review. Veterinarni Medicina, 53(8): 397-411.
Oliveira AH, Ogrodowski CC, Macedo ACD, Santana MHA and Gonçalves LR. (2013). Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production. Brazilian Journal of Microbiology, 44(4): 1097-1104.
O'Regan M, Martini I, Crescenzi F, De Luca C and Lansing M. (1994). Molecular mechanisms and genetics of hyaluronan biosynthesis. International Journal of Biological Macromolecules, 16(6): 283-286.
Osman MEA, El-Shouny W, Talat R and El-Zahaby H. (2012). Polysaccharides production from some Pseudomonas syringae pathovars as affected by different types of culture media. The Journal of Microbiology, Biotechnology and Food Sciences, 1(5): 1305.
Pires AM, Macedo AC, Eguchi SY and Santana MH. (2010). Microbial production of hyaluronic acid from agricultural resource derivatives. Bioresource Technology, 101(16): 6506-6509.
Shene C, Canquil N, Bravo S and Rubilar M. (2008). Production of the exopolysaccharides by Streptococcus thermophilus: Effect of growth conditions on fermentation kinetics and intrinsic viscosity. International Journal of Food Microbiology, 124(3): 279-284.
Shen JW, Shi CW and Xu CP. (2013). Exopolysaccharides from Pleurotus pulmonarius: fermentation optimization, characterization and antioxidant activity. Food Technology and Biotechnology, 51(4): 520.
Sri Lakshmi Ramya Krishna Kanamarlapudi and Sudhamani Muddada. (2017). Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30. Biomed Research International, 2017: 11.
Tu NH and Trang PT. (2013). Effects of rice-washing water on the hyaluronic acid production of Streptococcus thermophilus, In 4th International Conference on Biomedical Engineering in Vietnam, 168-170 p.
Vaningelgem F, Zamfir M, Adriany T and De Vuyst L. (2004). Fermentation conditions affecting the bacterial growth and exopolysaccharide production by Streptococcus thermophilus ST 111 in milk-based medium. Journal of Applied Microbiology, 97(6): 1257-1273.
Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH and Whitman W. (2009). Bergey's manual of systematic bacteriology. The Firmicutes, Springer Science and Business Media. 3.
Weisburg WG, Barns SM, Pelletier DA and Lane DJ. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173(2): 697-703.
Wang J, Zhao X, Tian Z, Yang Y and Yang Z. (2015). Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir. Carbohydrate Polymers, 125: 16–25.
Zhang Q, Ren J, Zhao H, Zhao M, Xu J and Zhao Q. (2011). Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. International Journal of Food Science and Technology, 46(5): 1014-1020.
Published
2017-09-27
How to Cite
Tabatabaei, D., & Sepahy, A. A. (2017). Comparative evaluation of hyaluronic acid production by Streptococcus thermophilus isolated from yoghurt. Journal of Research in Biology, 7(6), 2342-2354. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/453
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