Physicochemical, phytochemical and antioxidant studies on leaf extracts of Mallotus tetracoccus (Roxb.) Kurz
Keywords:
Mallotus tetracoccus, Antioxidant, DPPH, Total phenols, Phosphomolybdenum activity
Abstract
In this study quantitative determination of active phytochemicals, physicochemical properties and antioxidant studies of various extracts of Mallotus tetracoccus leaves were studied. The Mallotus tetracoccus leaf powder was extracted with different organic solvent such as petroleum ether, chloroform, ethyl acetate, acetone and aqueous ethanol. The total phenols and flavonoids content of various organic extracts were determined by folin-cicalteu method and aluminium chloride method. Other antioxidant activities were determined by DPPH free radical scavenging assay, FTC, TBA and phosphomolybdenum methods. Among the various extracts of Mallotus tetracoccus leaves, ethanol extract possessed high total phenolic contents (175.67±1.1 mg GAE/g). The reducing power assay values were in the range of 0.013±0.002 to 1.766±0.017 for the extracts with increasing concentrations. Thus the antioxidant activity of different extracts was in the order of ethanol > acetone > ethyl acetate > chloroform > petroleum ether extract. The findings showed that, among the various extracts of Mallotus tetracoccus, the organic polar extracts possessed high phenols and antioxidant activities.
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Arfan M, Amin H, Karamać M, Kosińska A, Amarowicz R, Shahidi F and Wiczkowski W. 2007. Antioxidant activity of extracts of Mallotus philippensis fruit and bark. Journal of Food Lipids, 14(3): 280–297.
Braca A, Sortino C, Politi M, Morelli I and Mendez J. 2002. Antioxidant activity of flavonoids from Licania licaniaeflora. Journal of Ethnopharmacology, 79(3): 379-381.
Chang CC, Yang MH, Wen HM and Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3): 178-182.
Chattopadhyay D, Arunachalam G, Mandal AB, Sur TK, Mandal SC and Bhattacharya SK. 2002. Antimicrobial and anti-inflammatory activity of folklore: Mallotus peltatus leaf extract. Journal of Ethnopharmacology, 82(2-3): 229–237.
Cheng XF, Chen Z and Zeng-Mu M. 1999. Two new diterpenoids from Mallotus apelta Muell. Arg. Journal of Asian Natural Products Research, 1(3): 319–325.
Cragg GM, Newman DJ. 2000. Antineoplastic agents from natural sources: achievements and future directions. Expert Opinion on Investigational Drugs, 9(12): 2783-2797.
Dasari NP, Ganga RB, Sambasiva RE, Mallikarjuna RT and Praneeth D. 2012. Quantification of phytochemical constituents and in vitro antioxidant activity of Synadium grantii. Free Radical and Antioxidant, 2(2): 68-72.
Dinis TC, Madeira VM and Almeida LM. 1994. Action of phenolic derivatives (acetoaminophen, salicilate and 5-aminosalicilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1): 161-169.
Halliwell B. 1997. Antioxidants and human diseases: a general introduction. Nutrition Reviews, 55(1 Pt 2): S44-S52.
Indian Pharmacopoeia. 1996. 4th ed. Ministry of health and family welfare, Govt. of India, New Delhi; Controller of publications: 1996. p. A-80.
Jaberian H, Piri K and Nazari J. 2013. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chemistry, 136(1): 237–244.
Jensen A. 1978. Chlorophyll and carotenoids. In: hallebust JA, craigie JS. (eds). handbook of physiochemical and biochemical Methods. Cambridge University Press, Cambridge, UK, 5-70 p.
Kalaivani T and Mathew L. 2010. Free radical scavenging activity from leaves of Acacia nilotica (L.) wild. ex delile an Indian medicinal tree. Food and Chemical Toxicology, 48(1): 298-305.
Khan RA, Khan MR, Sahreen S and Ahmed M. 2012. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chemistry Central Journal, 6: 12.
Kikuzaki H and Nakatani N. 1993. Antioxidant effects of some ginger constituents. Journal of Food Science, 58(6): 1407–1410.
Kim HS, Lim HK, Chung MW and Kim YC. 2000. Antihepatotoxic activity of bergenin, the major constituent of Mallotus japonicus, on carbon tetrachloride-intoxicated hepatocytes. Journal of Ethnopharmacology, 69(1): 79–83.
Lu F and Foo LY. 1995. Phenolic antioxidant component of evening primrose. In: nutrition, lipids, health and disease; Ong, ASH, Niki E, Packer L, Eds, American Oil Chemists Society Press: Champaign, Illinois.
Ma J, Jones SH and Hecht SM. 2004. A coumarin from Mallotus resinosus that mediates DNA cleavage. Journal of Natural Products, 67(9): 1614–1616.
Nurcholis W, Priosoeryanto BP, Purwakusumah ED, Katayama T and Suzuki T. 2012. Antioxidant, cytotoxic activities and total phenolic content of four Indonesian medicinal plants. Journal of Kimia Valensi, 2(4): 501-510.
Ottolenghi A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Archives of Biochemistry and Biophysics, 79: 355–358.
Prieto P, Pineda M and Aguilar M. 1999. Spectrophotometric quantitative of antioxidant capacity through the formation of a phosphomolybednum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2): 337-341.
Raja C, Biplab De, Nayakanti D, and Saikat S. 2012. Antiinflammatory, antinociceptive and antioxidant activities of Phyllanthus acidus L. extracts. Asian Pacific Journal of Tropical Biomedicine, 2(2): S953-S961.
Ramalakshmi S and Muthuchelian K. 2011. Analysis of bio-active constituents from the leaves of Mallotus tetracoccus (Roxb.) Kurz by gas chromatography-mass spectrometry. International Journal of Pharmaceutical Sciences and Research, 2(6): 1449-1454.
Ramalakshmi S and Muthuchelian K. 2012. Evaluation of antioxidant potential and antimicrobial studies of bark of medicinal plant, Mallotus tetracoccus (Roxb.) Kurz. Journal of Medicinal Plant Research, 6(38): 5156-5165.
Ramalakshmi S and Muthuchelian K. 2016. Development of nanodrug for treatment of breast cancer using Mallotus tetracoccus leaves - standardisation, synthesis and characterization. Nano Biomedicine and Engineering, 8(2): 82-90.
Siddhuraju P, Mohan PS and Becker K. 2002. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem, bark, leaves and fruit pulp. Food chemistry, 79(1): 61-67.
Siddhuraju R and Becker K. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa olifera Lam.) leaves. Journal of Agricultural and Food Chemistry, 51(8): 2144-2155.
SPSS. 2017. IBM Corp. Released 2017. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp.
Suba RN, Navanesan S, Sinniah SK, Wahab NA and Sim KS. 2012. Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complementary and Alternative Medicine, 12: 128.
Tanaka T, Ito T, Iinuma M, Takahashi Y and Naganawa H. 1998. Dimeric chalcone derivatives from Mallotus philippensis. Phytochemistry, 48(8): 1423–1427.
Published
2018-07-23
How to Cite
Subbiah, R., & Krishnaswamy, M. (2018). Physicochemical, phytochemical and antioxidant studies on leaf extracts of Mallotus tetracoccus (Roxb.) Kurz. Journal of Research in Biology, 8(5), 2518-2528. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/473
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