Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts
Keywords:
Ultraviolet B radiation, Ferulic acid, Human dermal fibroblasts, Inflammatory markers, Photoprotection
Abstract
Ultraviolet B (UVB 290-320 nm) participate in the development of the cutaneous inflammatory response which includes a cascade of events that involves increased expression of cyclooxygenase-2 (COX-2), release of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. Peroxisome proliferator-activated receptors (PPARα/γ) are considered to be potential targets for photo protection because they inhibit UVB mediated inflammatory responses. In this study, we investigated the effect of ferulic acid on UVB-radiation induced expression of TNF-α and COX-2 in human dermal fibroblasts (HDFa). Further, the action of ferulic acid on PPARα/γ activation and its binding interaction with these proteins were analyzed by induced fit docking. We found that onetime UVB exposure (19.8 mJ/cm2) showed significantly increased the expressions of COX-2 and TNF-α in HDFa after 4 h post-irradiation when compared to the control cells. Ferulic acid pretreatment for 30 min before UVB exposure prevented UVB-induced overexpression of these inflammatory markers. It has also been found that ferulic acid activates PPARα/γ expressions in HDFa. Further, induced fit docking analysis showed that there was a greater binding interaction of ferulic acid with PPARγ than PPARα. Thus, ferulic acid exhibits beneficial effects against UVB-induced inflammatory responses probably through down-regulating COX-2 and TNF-α expressions and activating PPARα/γ agonists.
References
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Ricote M, Li CA, Willson TM, Kelly CJ, Glass CK. 1998. The peroxisome proliferator-activated receptor-gamma is a negative regulator of macrophage activation. Nature. 391(6662): 79-82.
Saija A, Tomaino A, Trombetta D, Pasquale D, Uccella A, Barbuzzi T, Paolino D, Bonina F. 2000. In vitro and in vivo evaluation of caffeic and ferulic acids as topical photoprotective agents. Int. J. Pharm., 199(1): 39–47.
Schrodinger Suite. 2009. The induced Fit Docking protocol Schrodinger, LLC, New York, NY.
Sharma SD and Katiyar SK. 2010. Dietary grape seed proanthocyanidins inhibit UVB-induced cyclooxygenase-2 expression and other inflammatory mediators in UVB-exposed skin and skin tumors of SKH-1 hairless mice. Pharm. Res., 27(6): 1092-1102.
Waku TT, Shiraki T, Oyama, Yoshito Fujimoto, Kanako Maebara, Narutoshi Kamiya. 2009. “Structural insight into PPARγ activation through covalent modification with endogenous fatty acids,” J. Mol. Biol., 385(1):188–199.
Wallace AC, Laskowski RA, Thornton JM. 1995. LIGPLOT: A program to generate schematic diagrams of protein ligand interactions. Prot. Eng,. 8(2):127 34.
Zhang Q, Southall MD, Mezsick SM, Johnson C, Murphy RC, Konger RL Travers JB. 2004. Travers JB Epidermal peroxisome proliferator-activated receptor gamma as a target for ultraviolet B radiation. J .Biol. Chem., 280: 73-9.
Alexia GL, Richard LN, Sophie Gangloff C, Moncef G. 2003. Differential regulation of TNF- alpha, IL-6 and IL-10 in UVB-irradiated human keratinocytes via cyclic AMP/protein kinase A pathway. Cytokine, 23 (4-5) : 138–149.
Barone E, Calabrese V, Mancuso C. 2009. Ferulic acid and its therapeutic potential as a hormetin for age-related diseases. Biogerontology 10 (2): 97–108
Blanquart C, Barbier O, Fruchart JC, Staels B, Glineur C. 2003. Peroxisome proliferator –activated receptors: regulation of transcriptional activities and rules in inflammation. The journal of steroid biochemistry and molecular biology. 85 (2-5): 267–273.
Chilampalli C, Guillermo R, Zhang X, Kaushik RS, Young A, Zeman D, Hildreth MB, Fahmy H, Dwivedi C. 2011. Effects of magnonol on UVB-induced skin cancer development in mice and its possible mechanism of action. BMC Cancer 11, 456.
DeLano WL. 2002. PYMOL a molecular graphics system. DeLano Scientific: San Carlos, CA, USA. Web link: http://www.pymol.org
Glide. 2009. version 5.5, Schrodinger, LLC, New York, NY.
Gregoris E, Fabris S, Bertelle M, Grassato L, Stevanato R. 2011. Propolis as potential cosmeceutical sunscreen agent for its combined photoprotective and antioxidant properties. Int. J. Pharm., 405 (1-2):97-101.
Grimble Robert F. 2002. Inflammatory status and insulin resistance. Current Opinion in Clinical Nutrition & Metabolic Care 5 (5): 551–559.
Hirata A, Murakami Y, Atsumi T, Shoji M, Ogiwara T, Shibuya K, Ito S, Yokoe I, Fujisawa S. 2005. Ferulic acid dimer inhibits lipopolysaccharide-stimulated cyclooxygenase-2 expression in macrophages. InVivo., 19 (5): 849-53.
Hirsch EC, Breidert T, Rousselet E, Hunot S, Hartmann A, Michel PP.2003. The Role of Glial Reaction and Inflammation in Parkinson's Disease Ann. N. Y. Acad. Sci., 991: 214–228.
Kanagalakshmi A and Rajendra Prasad N. 2014. Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts. International Journal of Nutr. Pharmaco. Neurol. Dise., 4(4): 203-213.
Kim JK, Sukyeong M, Myung Suk K, Mi-Bo Kim, Bo-Kyung Sa and Jae-Kwan Hwang. 2012. 5,7-Dimethoxyflavone, an activator of PPARa⁄c, inhibitsUVB-induced MMP expression in human skin fibroblast cells. Exp. Dermatol., 21 (3): 211-216.
Ko A, Schwarz T, Kirnbauer R, Urbanski A, Ansel JC patricia p, john c. 1990. Human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. J .Exp. Med.,172(6) : 1609–1614.
Kondo S, Kono T, Sauder DN, McKenzie R. 1993. IL-8 gene expression and production in human keratinocytes and their modulation by UVB. J. Invest. Dermatol., 101(5): 690–694.
Kondo S. 1999. The roles of keratinocyte-derived cytokines in the epidermis and their possible responses to UVA-irradiation. J .Invest. Dermatol., 4 (2) :177– 183.
Lee C, Park GH, Ahn EM, Kim BA, Park C Jang JH. 2013. Protective effect of Codium fragile against UVB-induced pro-inflammatory and oxidative damages in HaCaT cells and BALB/c mice. Fitoterapia., 86: 54–63.
Lee CW, Ko HH, Lin CC, Chai CY, Chen WT, Yen F. 2013. Artocarpin attenuates ultraviolet B-induced skin damage in hairless mice by antioxidant and anti-inflammatory effect. Food. Chem. Toxicol., 60: 123–129.
Muthusamy V and Piva TJ. 2010. The UV response of the skin: a review of the MAPK, NF kappa B and TNF alpha signal transduction pathways. Arch Dermatol Res., 302: 5-17.
Oresajo C, Stephens T, Hino PD, Law RM, Yatskayer M, Foltis P, Pillai S, Pinnell SR. 2008. Protective effects of a topical antioxidant mixture containing vitamin C, ferulic acid, and phloretin against ultraviolet-induced photodamage in human skin. J. Cosmet. Dermatol., 7(4): 290–297.
Picone P, Bondi ML, Montana G, Bruno A, Pitarresi G, Giammona G, Di Carlo M. 2009. Ferulic acid inhibits oxidative stress and cell death induced by Ab oligomers: improved delivery by solid lipid nanoparticles. Free Radic. Res,. 43(11): 1133–1145.
Prasad NR, Karthikeyan S, Kanimozhi G, Mahalakshmi R. 2011. Radiosensitizing effect of ferulic acid on human cervical carcinoma cells in vitro Toxicol.in Vitro., 25(7): 1366–1375
Prasad NR, Ramachandran S, Pugalendi KV, Menon VP. 2007. Ferulic acid inhibits UV-B–induced oxidative stress in human lymphocytes Nutr. Res., 27 (7): 559–564.
Ramachandran S and Rajendra Prasad N. 2008. Effect of ursolic acid, a triterpenoid antioxidant, on ultraviolet-B radiation-induced cytotoxicity, lipid peroxidation and DNA damage in human lymphocytes. Chem. Biolo. Inter., 176(2-3): 99–107.
Ramachandran S and Rajendra Prasad N. 2012. Sesamol modulates ultraviolet-B-induced apoptotic and inflammatory signaling in human skin dermal fibroblasts. Nutr. Pharmaco. Neurol. Dise., 2(1): 31-39.
Ramachandran S, Rajendra Prasad N, Karthikeyan S. 2010. Sesamol inhibits UVB-induced ROS generation and subsequent oxidative damage in cultured human skin dermal fibroblasts. Arch Dermatol Res. 302: 733-44.
Ricote M, Li CA, Willson TM, Kelly CJ, Glass CK. 1998. The peroxisome proliferator-activated receptor-gamma is a negative regulator of macrophage activation. Nature. 391(6662): 79-82.
Saija A, Tomaino A, Trombetta D, Pasquale D, Uccella A, Barbuzzi T, Paolino D, Bonina F. 2000. In vitro and in vivo evaluation of caffeic and ferulic acids as topical photoprotective agents. Int. J. Pharm., 199(1): 39–47.
Schrodinger Suite. 2009. The induced Fit Docking protocol Schrodinger, LLC, New York, NY.
Sharma SD and Katiyar SK. 2010. Dietary grape seed proanthocyanidins inhibit UVB-induced cyclooxygenase-2 expression and other inflammatory mediators in UVB-exposed skin and skin tumors of SKH-1 hairless mice. Pharm. Res., 27(6): 1092-1102.
Waku TT, Shiraki T, Oyama, Yoshito Fujimoto, Kanako Maebara, Narutoshi Kamiya. 2009. “Structural insight into PPARγ activation through covalent modification with endogenous fatty acids,” J. Mol. Biol., 385(1):188–199.
Wallace AC, Laskowski RA, Thornton JM. 1995. LIGPLOT: A program to generate schematic diagrams of protein ligand interactions. Prot. Eng,. 8(2):127 34.
Zhang Q, Southall MD, Mezsick SM, Johnson C, Murphy RC, Konger RL Travers JB. 2004. Travers JB Epidermal peroxisome proliferator-activated receptor gamma as a target for ultraviolet B radiation. J .Biol. Chem., 280: 73-9.
Published
2014-11-17
How to Cite
A, K., B, A., S, M., D, A., D, V., R, K., M, G., G, S., & N, R. P. (2014). Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts. Journal of Research in Biology, 4(8), 1505-1515. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/509
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Articles
