Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts

  • Kanagalakshmi A Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Agilan B Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Mohana S Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Ananthakrishnan D Bioinformatics Infrastructure Facility (BIF),University of Madras, Chennai-25
  • Velmurugan D Bioinformatics Infrastructure Facility (BIF),University of Madras, Chennai-25
  • Karthikeyan R Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Ganesan M Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Srithar G Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
  • Rajendra Prasad N Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India
Keywords: Ultraviolet B radiation, Ferulic acid, Human dermal fibroblasts, Inflammatory markers, Photoprotection

Abstract

Ultraviolet B (UVB 290-320 nm) participate in the development of the cutaneous inflammatory response which includes a cascade of events that involves increased expression of cyclooxygenase-2 (COX-2), release of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. Peroxisome proliferator-activated receptors (PPARα/γ) are considered to be potential targets for photo protection because they inhibit UVB mediated inflammatory responses. In this study, we investigated the effect of ferulic acid on UVB-radiation induced expression of TNF-α and COX-2 in human dermal fibroblasts (HDFa). Further, the action of ferulic acid on PPARα/γ activation and its binding interaction with these proteins were analyzed by induced fit docking. We found that onetime UVB exposure (19.8 mJ/cm2) showed significantly increased the expressions of COX-2 and TNF-α in HDFa after 4 h post-irradiation when compared to the control cells. Ferulic acid pretreatment for 30 min before UVB exposure prevented UVB-induced overexpression of these inflammatory markers. It has also been found that ferulic acid activates PPARα/γ expressions in HDFa. Further, induced fit docking analysis showed that there was a greater binding interaction of ferulic acid with PPARγ than PPARα. Thus, ferulic acid exhibits beneficial effects against UVB-induced inflammatory responses probably through down-regulating COX-2 and TNF-α expressions and activating PPARα/γ agonists.

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Published
2014-11-17
How to Cite
A, K., B, A., S, M., D, A., D, V., R, K., M, G., G, S., & N, R. P. (2014). Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts. Journal of Research in Biology, 4(8), 1505-1515. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/509