Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC
Keywords:
Eucalyptus gomphocephala, quantitative analysis, qualitative analysis, aqueous extract, gallic acid, Pseudomonas aeruginosa
Abstract
This work aims to find out the molecule responsible for the good activity against the strains of Pseudomonas aeruginosa reflected in a pre work done on the phytochemical study of aqueous extract of Eucalyptus gomphocephala. We have initially processed the quantitative colorimetric determination by a UV-Vis spectrophotometer for total polyphenols and flavonoids and a qualitative analysis by high performance liquid chromatography (HPLC) coupled with mass spectroscopy. Quantitative determinations of total polyphenols by the Folin-Ciocalteu reagent and flavonoids by AlCl3 method revealed the richness of this extract in total polyphenols. Qualitative analysis by HPLC / ESI-MS revealed the presence of gallic acid. This molecule was tested by the agar diffusion method and the macrodilution method in liquid medium, which showed greater activity than the aqueous extract. The results obtained in this study suggest that the gallic acid may be used in the treatment of infections caused by Pseudomonas aeruginosa.
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Singh IP, Umehara K and ETOH H. 1999. Macrocarpals in Eucalyptus sp. As attachment- inhibitors against the blue mussel. Natural Product Letters. 14(1): 11-15.
Wong SP, Leong LP and William Koh JH. 2006. Antioxidant activities of extracts of selected plants. Food chemistry. 99(4): 775-783
Al-Sayed E, Martiskainen O, Bobrowska-Hägerstrand M, Sinkkonen J, Törnquist K, Pihlaja K, Ayoub N and Singab AN. 2010. Phenolic compounds from Eucalyptus gomphocephala with potential cytotoxic and antioxidant activities. Natural Product Communications. 5(10): 1639-1642.
Al-Sayed E, Singab A, Ayoub N, Martiskainen O, Sinkkonen J and Pihlaja K. 2012. HPLC–PDA–ESI–MS/MS profiling and chemopreventive potential of Eucalyptus gomphocephala DC. Food Chemistry. 133(3): 1017–1024.
Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Vasseur J, Cazin JC, Pinkas, M, Luyckx M and Cazin M. 1996. Oxygen species scavenging activity of phenolic extracts from hawthron fresh plant organs and pharmaceutical preparations. Arzneimittel-Forschung. 46(11): 1086-1089.
Barbouche N, Jeridi S and Ben Dhaou S. 2007. Alternative de lutte biologique contre Sitophilus oryzae (Coleoptera : curculionidae) par l’utilisation des huiles essentielles et des poudres de plantes. Rev. INAT. Revue de l'Institut National Agronomique de Tunisie (TN) 22(1): 85-96.
Boudy P. 1952. Guide du forestier en Afrique du nord. Ed. La Maison Rustique. Paris. 505
Bouharb H, K EL Badaoui, T Zair, H Shisseh, S Chakir and Alaoui T. 2014. Antibacterial Evaluation and Phytochemical Screening of Eucalyptus gomphocephala DC against Pseudomonas aeruginosa. Asian Journal of Pharmaceutical and Clinical Research. 7(4): 264-267
Ebrahimzadeh MA, Pourmorad F and Bekhradnia AR. 2008. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. African Journal of Biotechnology. 7(18): 3188-3192.
Elliot WR and Jones DL. 1983. Encyclopedia of Australian Plants suitable for Cultivation. 4:528.
Ferrari J. 2002. Contribution à la connaissance du métabolisme secondaire des Thymelaeaceae et investigation phytochimique de l’une d’elles: Gnidia involucrata Steud. ex A. Rich. Thèse de doctorat. Lausanne, Faculté des sciences 228
Guendouz BA and Doumandji-Mitiche B. 2006. Effect of plant extract of Eucalyptus gomphocephala, Shinus molle and Melia azedarach on Schistocerca gregaria (FORSKÅL 1775) (Insectaorthoptera) Under Laboratory conditions. Ninth Arab Congress of Plant Protection, 19-23 November, Damascus, Syria
Hyodo S, Etoh H, Yamashita N, Sakata K and Ina K. 1992. Structure of resinosides from Eucalyptus resinifera as repellents against the blue mussel, Mytilus edulis. Bioscience Biotechnology and Biochemistry. 56(1): 138.
Ikuro A, Kenji K and Hiroshi N. 2000. Antioxidative galloyl esters as enzyme inhibitors of p-hydroxybenzoate hydroxylase. FEBS Letters. 483: 131-134.
IPD. 1996. Indian Pharmacopoeia. Delhi: Government of India, Ministry of Health and Family Welfare-Controller of Publications. 11: 310.
Ksouri R, Megdiche W, Falleh H, Trabelsi N, Boulaaba M, Smaoui A and Abdelly C. 2008. Influence of biological, environmental and technical factors on phenolic content and antioxidant activities of Tunisian halophytes. Comptes Rendus biologies. 331(11): 865-873
Ksouri R, Ksouri MW, Jallali I, Debez A, Magné C, Hiroko I and Chedly A. 2012. Medicinal halophytes: potent source of health Références bibliographiques112 promoting biomolecules with medical, nutraceutical and food applications. Critical Reviews in Biotechnology. 32 (4): 289-326
Kuntie V, Pejie N, Ivkovie B, Vugie Z, Ilie K, Mieie S and Vukojevie V. 2007. Isocratic R-PHPLC method for rutin determination in solid oral dosage forms. Journal of pharmaceutical and biomedical analysis. 43(2): 718-721.
Maisuthisakul P, Pongsawatmanit R and Maitree Suttajit. 2007. Assessment of phenolic content and free- radical scavenging capacity of some Thai indigenous plants. Food Chemistry. 100(4): 1409-1418.
Marien JN. 1993. Projet d’amélioration des Eucalyptus au Maroc. Afocel ed. 114
Premkumar J, Sakharkar MK, Chu Sing L, Thean Hock T and Kishore RS. 2010. Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro. International Journal of Biological Sciences. 6(6): 556–568.
Premkumar J, Kishore RS, Chu Sing L, Vincent TKC and Sakharkar MK. 2011. Insights into antifolate activity of phytochemicals against Pseudomonas aeruginosa. Journal of Drug Targeting. 19 (3): 179-188
Singh AK, Monika K and Sushil K. 1999. Non-volatile constituents of Eucalyptus: A review on chemistry and biological activities. Journal of Medicinal Aromatic Plant Sciences. 21(2): 375– 407.
Singh IP, Umehara K and ETOH H. 1999. Macrocarpals in Eucalyptus sp. As attachment- inhibitors against the blue mussel. Natural Product Letters. 14(1): 11-15.
Wong SP, Leong LP and William Koh JH. 2006. Antioxidant activities of extracts of selected plants. Food chemistry. 99(4): 775-783
Published
2014-12-20
How to Cite
Bouharb, H., El Badaoui, K., Amechrouq, A., & El Amri, J. (2014). Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC. Journal of Research in Biology, 4(8), 1549-1556. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/514
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