Screening and isolation of potent biosurfactant producing Bacillus subtilis CS14 from contaminated soil samples of Kanchipuram, India
Abstract
A good biosurfactant must reduce the surface tension of the water from 72 to 35 milli-Neuton/meter (mN/m) and should show a surface activity of at least 37 mN/m. An extensive screening with 13 contaminated soils and one oil refinery sludge was carried out to isolate a potential strain.. Totally 3662 colonies were obtained from all 14 contaminated soil samples over a period of three months from which 212 morphologically different colonies were tested for biosurfactant production and 22 positive strains were identified. All the isolates were tested quantitatively and the isolate CS14 showed maximum surface activity of 51.38 mN/m. Taxonomic identification of the biosurfactant producing strain CS14 was performed using 16s rDNA studies and was identified as Bacillus subtilis.
References
Altschul F. Stephen, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller and David J. Lipman, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25(17):3389-3402.
Ashtaputre AA, Shah AK. 1995. Emulsifying property of a viscous exopolysaccharide from Sphingomonas paucimobilis. World J. Microbiol. Biotechnol., 11(2):219 -222.
Bento Fatima Menezes, Flavio A. de Oliveira Camargo, Benedict C. Okeke and William T. Frankenberger Jr. 2005. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. J. Microbiol. Res., 160:249-255.
Bodour A. Adria, Kevin P. Dress and Raina M. Maier. 2003. Distribution of biosurfactantproducing bacteria in undisturbed and contaminated arid southwestern soils. J. Appl. Env. Microbiol., 69 (6):3280-3287.
Cappuccino JG and Sherman N. 1999. Microbiology: A Laboratory Manual (6th Ed) Pearson Education publication, Singapore.
Carillo PG, Mardaraz SI, Pitta-Alvarez and Giulietti AM. 1996. Isolation and section of biosurfactant producing bacteria. World J. Microbiol. Biotechnol., 82-94.
De-Souza T. Jorge, Marjan De Boer, Pieter De Waard, Teris A. van Beek and Jos M. Raaijmakers. 2003. Biochemical, genetic, and zoosporicidal properties of cyclic lipopeptide surfactants produced by Pseudomonas fluorescens. Appl.Env. Microbiol., 69(12):7161-7172.
Gnanamani A, Kavitha V, Radhakrishnan N, Mandal AB. 2010. Bioremediation of Crude Oil Contamination Using Microbial Surface-Active Agents: Isolation, Production and Characterization. J. Bioremed. Biodegrad., 1:107-115.
Gunther W. Nereus, Alberto Nunez, William Fett and Daniel KY, Solaiman, 2005. Production of rhamnolipids by Pseudomonas chlororaphis, a nonpathogenic bacterium. Appl. Env. Microbiol., 71(5):2288-2293.
Heuer H, Krsek M, Baker P, Smalla K, Wellington HME. 1997. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel electrophoretic separation in denaturing gradients. Appl. Env. Microbiol., 63:3233-3241.
Holt JG NR, Krieg PHA, Sneath JT, Staley and Williams ST. 1994. Bergey’s manual of determinative bacteriology. Williams and Wilkins. Baltimore, USA. 1-4.
Huang SH, Bing P, Zhi hui Y, Li yuan C and Li cheng Z. 2008. Chromium Accumulation, Microorganism Population and Enzyme Activities in Soils around Chromium-Containing Slag Heap of Steel Alloy Factory, Transactions Of Nonferrous Metal Society Of China. 19:241-248.
Jacobucci DFC, Oriani MRG, Durrant LR. 2009. Reducing COD level on oily effluent by utilizing biosurfactant-producing bacteria. Braz. Arch. Biol. Technol., 52:1037-1042.
Jiang R, Huang S, Chow TA and Yang J. 2009. Nitric oxide removal from flue gas with a biotrickling filter using Pseudomonas putida. J. Hazard. Mater., 164:432-441.
Mishra S, Doble M. 2008. Noval chromium tolerant microorganisms: isolation, characterization and their bioreduction capacity, Ecotoxicol. Env. Safety 71:874-879.
Mukherjee S, Das P and Sen R. 2006. Towards commercial production of microbial surfactants. Trends Biotechnol., 24:509-515.
Mulligan CN. 2004. Environmental applications for biosurfactants. Environ. Poll., 7:362-366.
Muthusamy Krishnaswamy, Subbuchettiar Gopalakrishnan, Thiengungal Kochupappy Ravi and Panchaksharam Sivachidambaram, 2008. Biosurfactants: Properties, commercial production and application. Curr. Sci., 94(6):736-747.
Ochsner A. Urs, Andreas K. Koch, Armin Fiechter and Jakob Reiser. 1994. Isolation and characterization of a regulatory gene affecting rhamonolipid biosurfactant synthesis in Pseudomonos aeruginosa. J.Bacteriol., 176(7):2004-2054.
Ramesh Balasubramanian, Kuppuswamy Kavitha, Gopalakrishnan Suresh, Balakrishnan Usharani, Nagaiya Ravichandran and Ganesan Vijaiyansiva. 2010. Studies on Distribution of Biosurfactant Producing Bacteria in Contaminated and Undisturbed Soils of Kanchipuram. Australian J. Basic Appl. Sci., 4(9):4429-4434.
Reiling HE, Thanei-wyss U, Guerra-santos LH, Hirt R, Kappel O and Fiechter A. 1986. Pilot plant production of rhamnolipid biosurfactnt by Pseudomonas aeruginosa. Appl. Env. Microbiol., 51 (5):985-989.
Rodrigues LR, Teixeria JA, Mei HC. 2006. Oliveria Physicochemical and Functional Characterization of a Biosurfactant Produced by Lactococcus lactis 53. Colloids and Surfaces B: Biointerfaces 49:79-86.
Sabesan R, Dhanalakshmi A, William A and Thangaraj K. 2002. Text book of allied Physics. Popular Book Depot. Second Reprint. 65-68.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M and Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biol. Evol. (In Press).
Xu J, Millar BC, Moore JE, Murphy K, Webb H, Fox AJ, Cafferkey M and Crowe MJ. 2003. Employment of broad-range 16S rRNA PCR to detect aetiological agents of infection from clinical specimens in patients with acute meningitis – rapid separation of 16S rRNA PCR amplicons without the need for cloning. J. Appl. Microbiol., 94:197-206.
Youssef Noha H, Kathleen E. Duncan, David P. Nagle, Kristen N. Savage, Roy M. Knapp and Michael J. McInerney, 2004. Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Meth., 56(3):339-347.
