Standardized protocol for the in vitro culture of Artemisia annua L. – A medicinal plant at high altitudes of Nilgiris, the Western Ghats

  • Paulsamy S Department of Botany, Kongunadu Arts and Science College, Coimbatore 641 029, Tamil Nadu, India
  • Ganesan CM Department of Botany, Kongunadu Arts and Science College, Coimbatore 641 029, Tamil Nadu, India
Keywords: In vitro culture, Medicinal plant, Artemisia annua, Nilgiris, Western Ghats,, India

Abstract

A reliable protocol for callus induction and organogenesis, and successful plantlet survivability through hardening were developed for leaf explants of the medicinal plant species, Artemisia annua L. MS medium containing the auxin, NAA at 0.9mg/l is determined to be the optimum concentration for callus induction. Higher shooting (20.67/callus) was performed in the medium supplemented with BAP and GA3 at 0.5 and 1.0 mg/l respectively while subculturing the callus. Maximum number of roots (12.00 roots/shoot) was noted to be obtained in the medium containing IBA at 0.9mg/l. The in vitro regenerated plantlets were successfully acclimatized (86% survivability rate) in the hardening medium encomposing vermiculate, coir waste and forest litter in the ratio of 1:1:1 by volume

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Published
2011-07-12
How to Cite
Paulsamy S, & CM, G. (2011). Standardized protocol for the in vitro culture of Artemisia annua L. – A medicinal plant at high altitudes of Nilgiris, the Western Ghats. Journal of Research in Biology, 1(3), 173-178. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/63