Isolation, screening and induction of mutation in strain for extra cellular lignin peroxidase producing bacteria from soil and its partial purification
Abstract
The Lignin peroxidase enzyme production was carried out through bacteria isolated from sample soil collected around decayed wood at Sulur, Coimbatore. In this report, a mutant of Bacillus subtilis LPTK was obtained after chemical mutagenesis with EMS and screened for better Lignin peroxidase production. In the production optimization studies, the bacterial strain needs temperature around 37OC, pH.6, lactose as a carbon source, peptone as nitrogen source and incubation time for 36 hours for its higher enzyme productivity. The partial purification was also done
References
Ahammed S and Prema P. 2002. Influence of media nutrients on synthesis of lignin peroxidase from Aspergillus sp., Appl Biochem Biotechnol, 102-103(1-6):327-36.
Radwan and Dora SA. 2010. Successive Construction of β-Glucosidase Hyperproducers of Trichoderma Harzianum Using Microbial Biotechnology Techniques, J. Microbial & Biomedical Technology 2:070-073.
Bradford MM. 1976. A rapid and sensitive for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-254.
Bo Zhang, Hua-Jiang Huang and Shri Ramaswamy. 2008. Reaction Kinetics of the Hydrothermal Treatment of Lignin, J. Applied Biochemistry and Biotechnology 147:119-131.
Elba P S. Bon, Hilton J Nasecimento, jacyara M, B Macedo and Jose G, silva Jr. 1999. Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure, Biotechnology Techniques 13:289-293.
Haemmerli SD, Leilosa MSA, Sanglard D and Fiechter A. 1996. Oxidation of benzo(a) pyrene by extracellular ligninases of phanerochaete chrysossporium: veratyl alcohol and stability of ligninases, J. Biochem, 261:6900-6903.
Kirk TK and Farrell. 1987. Enzymatic combustion: the microbial degradation of lignin, ann, ERv, Microbiol, 41:465-505.
Korsten L and Cook N. 1996. Optimizing culturing conditions for bacillus subtilis, South African Avocado Growers Association Yearbook, 19:54-58.
Kotchoni SO and Shonukan OO. 2002. Regualtory mutations affecting the synthesis of cellulase, World J Microbiol Biotechnol., 18:487- 491.
Ramachandra M, Crawford DL and Hertel G. 1988. Characterization of an extracellular lignin peroxidase of the lignocellulolytic actinomycete, streptomyces and viridosporus, App. Environ, Microbiol., 54:3057-3064.
Tien M, Kersten PJ, Kalyanaraman B and Kirk TK. 1985. The ligninase of Phanerochaete chrysosporium generates cation redicals from methoxybenzenes. J Biol Chem, 10:260(5):2609- 2612.
Tien M, Kirk TK, Bull C and Fee JA. 1986. Steady state and transient state kinetics on the oxidation of 3, 4-dimethoxybenzyl alcohol catatyzed by the ligninase of phanerocheate chrysosporium burds, J. Biol Chem, 261(4):1687- 1693.
Tuncer M, Kurul A, Isikli M and elenk FGC. 2004. Optimization of extracellular endoxylanase, endoglucanase and peroxidase production by strptomyces sp. F2621 isolated in turkey, J. Applied Microbiology 97:783-791.
Yeoh teik loon. 2006. Screening of lignin degrader from soil, thesis. Submitted for the degree of bachelor of chemical engineering.
Zolta’n pragai and Colin R Harwood. 2002. Regulatory interactions between the pho and rBdependent general stress regulations of bacillus subtilis 148:1593-1602.
