Kinetics of dose-response relationship of heavy metals with dehydrogenase activity in wastewater bacteria
Keywords:
Dehydrogenase activity, heavy metals, dose-response models, toxicity
Abstract
Toxicity of Zn2+, Cd2+ and Co2+ to Escherichia coli, Pseudomonas and Bacillus species isolated from petroleum refinery effluent was assessed using dehydrogenase activity (DHA) inhibition test. Exposure of the cells to the metal ions resulted in inhibition of dehydrogenase activity. The median inhibitory concentration of the metal ions ranged from 0.0554 to 0.3883 mM (Zn2+), 0.0279 to 0.3004 mM (Cd2+) and 0.0013 to 0.2778 mM (Co2+). The trends of the inhibitory effects could be mathematically described with logistic and sigmoid dose-response models and in a manner similar to the non-competitive inhibition of enzymes. The threshold concentration above which toxic effect is observed ranged from 0.0013 mM (Zn2+ against Pseudomonas sp. RWW2) to 0.05 mM (Zn2+ against Escherichia coli). In terms of non-competitive inhibition of dehydrogenase activity, the threshold concentration ranged from 0.0183 mM (Cd2+ against Pseudomonas sp. DAF1) to 0.05 mM (Zn2+ against Escherichia coli). The coefficients of inhibition Ki correlated with the IC50, thus they are suitable parameters for kinetic analyses of metal toxicity against bacteria.
References
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Orji JC, Nweke CO, Nwabueze RN, Anyaegbu B, Chukwu JC, Chukwueke CP and Nwanyanwu CE. 2008. Impact of some divalent cations on periplasmic nitrate reductase and dehydrogenase enzymes of Escherichia, Pseudomonas and Acinetobacter species. Revista Ambi-Água 3(2):5-18.
Pérez-Garcia A, Codina JC, Cazoria FM and de Vicente A. 1993. Rapid respirometric toxicity test: sensitivity to metals. Bull. Environ. Contam. Toxicol., 50:703-708.
Purves D. 1985. Trace Elements Contamination of the Environment. Elsevier, Armsterdam.
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Stohs SJ and Bagchi D. 1995. Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med., 18:321-336.
Sugarman B. 1983. Zinc and infection. Rev. Infect. Dis., 138:147.
Surowitz KG, Titus JA and Pfister RM. 1984. Effect of cadmium accumulation on growth and respiration of a cadmium-sensitive strain of Bacillus subtilis and a selected cadmium-resistant mutant. Arch. Microbiol., 140:107-112.
Wang A and Crowley DE. 2005. Global gene expression responses to cadmium toxicity in Escherichia coli. J. Bacteriol., 187:3259-3266.
Binning K and Baird D. 2001. Survey of heavy metals in the sediments of the Swartkops river estuary, Port Elizabeth, South Africa. Water SA. 27(4):461-466.
Bitton G, Dutton R and Koopman B. 1988. Cell permeability to toxicants: an important parameter in toxicity tests using bacteria. CRC Crit. Rev. Environ. Cont., 18:177-187.
Bitton G and Koopman B. 1986. Biochemical tests for toxicity screening: In: Bitton G, Dutka BJ (eds). Toxicity Testing Using Microoganisms Vol l. CRC Press, Boca Raton, FL.
Bitton G, Koopman B and Agami O. 1992. MetPADTM: a bioassay for rapid assessment of heavy metal toxicity in wastewater. Water Environ. Res., 64:834-836.
Codina JC, Pérez-Garcia A and de Vicente A. 1994. Detection of heavy metal toxicity and genotoxity in wastewaters by microbial assay. Wat. Sci. Tech., 30(10):145-151.
Codina JC, Pérez-Garcia A, Romero P and de Vicente A. 1993. A comparison of microbial bioassays for the detection of metal toxicity. Arch. Environ. Contam. Toxicol., 25:250-254.
Coello Oviedo MD, Sales Márquez D and Quiroga Alonso JM. 2002. Toxic effects of metals on microbial activity in the activated sludge process. Chem. Biochem. Eng. Q. 16(3):139-144.
Davies CA, Tomlinson K and Stephenson T. 1991. Heavy metals in river tees estuary sediments. Environ. Technol., 12:961-972.
Doelman P, Jansen E, Michels M and van Til M. 1994. Effects of heavy metals in soil on microbial diversity and activity as shown by the sensitivity-resistance index, an ecologically relevant parameter. Biol. Fertil. Soil 17:177-184.
Gadd GM. 1993. Interactions of fungi with toxic metals. New Phytol., 124:25-60.
Goyer RA. 1997. Toxic and essential metal interactions. Annu. Rev. Nutri., 17:37-50.
Griffiths RP. 1983. The importance of measuring microbial enzymatic functions while assessing and predicting lond-term anthropogenic perturbations. Mar. Pollut. Bull., 14:162-165.
Guckert JB. 1996. Toxicity assessment by community analysis. J. Microbiol., Methods 25:101-112.
Hassen A, Saidi N, Cherif M and Boudabous A. 1998a. Resistance of environmental bacteria to heavy metals Bioresource Technol., 64:7-15.
Hassen A, Saidi N, Cherif M and Boudabous A. 1998b. Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thuringiensis. Bioresource Technol., 65:73-82.
Horsfall M and Spiff AS. 2002. Distribution and partitioning of trace metals to sediment of the lower reaches of the New Calabar River, Port Harcourt, Nigeria. Environ. Monitor. Assess., 78:309-326.
Ji G and Silver S. 1995. Bacterial resistance mechanism for heavy metals of environmental concern. J. Ind. Microbiol., 14:64-168.
Juliastuti SR, Baeyens J and Creemers C. 2003a. Inhibition of nitrification by heavy metals and organic compounds: The ISO 9509 test. Environ. Eng. Sci., 20(2):79-90.
Juliastuti SR, Baeyens J, Creemers C, Bixio B and Lodewyckx E. 2003b. The inhibition effects of heavy metals and organic compounds on the net maximum specific growth rate of the autotrophic biomass in activated sludge. J. Hazard. Mater., 100:71-283.
Kasahara M and Anraku Y. 1974. Succinate and NADH oxidase systems of Escherichia coli membrane vesicles: mechanism of selective inhibition of the system by zinc ions. J. Biochem., 76:967-976.
Kleiner D. 1978. Inhibition of respiratory system in Azotobacter vinelandi by divalent metal ions. FEBS Lett., 96(2):366-368.
Kobayashi M and Shimizu S. 1998. Metalloenzyme nitrile hydratase: structure, regulation and application to biotechnology. Nat. Biotechnol., 16:733-736.
Kroiss H, Schweighoffer P, Frey W and Matsche N. 1992. Nitrification Inhibition: a source identification method for combined municipal and industrial wastewater treatment plant. Water Sci. Technol., 26(5/6):1135-1146.
Li F and Tan TC. 1994. Effects of heavy metal ions on the efficacy of a mixed bacilli BOD sensor. Biosens. Bioelectron., 9:315-324.
Montuelle B, Latour X, Volat B and Gounot A-M. 1994. Toxicity of heavy metals to bacteria in sediments. Bull. Environ. Contam. Toxicol., 53:753-758.
Nies DH. 1999. Microbial heavy metal resistance. Appl. Microbiol. Biotechnol., 51:730-750.
Nwanyanwu CE and Abu GO. 2011. Assessment of viability responses of refinery effluent bacteria after exposure to phenol. J. Res. Biol. 8:594 -602.
Nweke CO. 2009. Kinetics of zinc toxicity to environmental bacterial isolates. Revista Ambi-Água 4:23-34.
Nweke CO, Okolo JC, Nwanyanwu CE and Alisi CS. 2006. Response of planktonic bacteria of New Calabar River to zinc stress. Afr. J. Biotechnol., 5(8):653-658.
Nweke CO, Alisi CS, Okolo JC and Nwanyanwu CE. 2007a. Toxicity of zinc to heterotrophic bacteria from a tropical river sediment. Appl. Ecol. Environ. Res., 5(1):123-132.
Nweke CO, Ntinugwa C, Obah IF, Ike SC, Eme GE, Opara EC, Okolo JC and Nwanyanwu CE. 2007b. In vitro effects of metals and pesticides on dehydrogenase activity in microbial community of cowpea (Vigna unguiculata) rhizoplane. Afr. J. Biotechnol., 6(3):290-295.
Nweke CO and Okpokwasili GC. 2010a. Influence of exposure time on phenol toxicity to refinery wastewater bacteria. J. Environ. Chem. Ecotoxicol., 2(2):20-27.
Nweke CO and Okpokwasili GC. 2010b. Inhibition of dehydrogenase activity in petroleum refinery wastewater bacteria by phenolic compounds. Revista Ambi. Agua 5(1):6-16.
Obst U, Holzapfel-Pschorn A and Wiegand-Rosinus M. 1988. Application of enzyme assays for toxicological water testing. Tox. Assess., 1:81-91.
Odum EP. 1985. Trends expected in stressed ecosystems. Bioscience 35:419-422.
Orji JC, Nweke CO, Nwabueze RN, Anyaegbu B, Chukwu JC, Chukwueke CP and Nwanyanwu CE. 2008. Impact of some divalent cations on periplasmic nitrate reductase and dehydrogenase enzymes of Escherichia, Pseudomonas and Acinetobacter species. Revista Ambi-Água 3(2):5-18.
Pérez-Garcia A, Codina JC, Cazoria FM and de Vicente A. 1993. Rapid respirometric toxicity test: sensitivity to metals. Bull. Environ. Contam. Toxicol., 50:703-708.
Purves D. 1985. Trace Elements Contamination of the Environment. Elsevier, Armsterdam.
Ren S and Frymier PD. 2003. Kinetics of the toxicity of metals to luminescent bacteria. Adv. Environ. Res., 7:537-547.
Stohs SJ and Bagchi D. 1995. Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med., 18:321-336.
Sugarman B. 1983. Zinc and infection. Rev. Infect. Dis., 138:147.
Surowitz KG, Titus JA and Pfister RM. 1984. Effect of cadmium accumulation on growth and respiration of a cadmium-sensitive strain of Bacillus subtilis and a selected cadmium-resistant mutant. Arch. Microbiol., 140:107-112.
Wang A and Crowley DE. 2005. Global gene expression responses to cadmium toxicity in Escherichia coli. J. Bacteriol., 187:3259-3266.
Published
2012-06-08
How to Cite
CO, N., & GC, O. (2012). Kinetics of dose-response relationship of heavy metals with dehydrogenase activity in wastewater bacteria. Journal of Research in Biology, 2(4), 392-402. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/230
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