Peanut Oil Cake: A Novel Substrate for Enhanced Cell Growth and Prodigiosin Production from Serratia marcescens CF-53
Abstract
Different agro-wastes such as peanut oil cake (POC), coconut oil cake (COC), sesame oil cake (SOC), safflower seed oil cake (SFOC) and cotton seed oil cake (CSOC) were screened for prodigiosin production through fermentation employing Serratia marcescens-CF-53. POC was highly beneficial for the pigment production. Fermentation parameters have been standardized; maximum amount of pigment was obtained (~40 mg ml-1) in POC extract at 30oC for 42 h using 8% inoculum density (1X107cell/ml) compared to PG broth (14.2 mg ml-1). The pigment yield was almost three fold higher than that of the PG broth. Use of POC extract as a raw material for pigment production could be of great commercial significance. This report appears to be the first one on prodigiosin production using POC as a substrate.
References
Beatriz Montaner, Ricardo Perez-Tomas. 2001. Prodigiosin-induced apoptosis in human colon cancer cells, Life sciences 68:2025-2036.
Cang S, Sananda M, Johdo O, Ohta, S, Nagamatsu Y, Yoshimoto A. 2000. High production of prodigiosin by Serratia marscecens grown on ethanol. Biotechnol. Lett. 22:1761-1765.
D’Aoust JY, Gerber NN. 1974. Isolation and purification of prodigiosin from Vibrio psychroerythrus. J. Bacteriol., 118:756-757.
Furstner A. 2003. Chemistry and biology of roseophilin and the prodigiosin alkaloids: a survey of the last 2500 years. Angew. Chem. Int. Ed., 42:3582-3603.
Fuqua C, Winans SC, Greenberg EP, 1996. Census and consensus in bacterial ecosystem: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu. Rev. Microbiol., 50:727-751.
Gerber NN. 1975. Prodigiosin like pigments. CRC Crit. Rev. Microbiol., 3:469-485.
Giri AV, Anandkumar N, Muthukumaran G, Pennathur G. 2004. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol., 4:1-10.
Haddix PL and Werner TF. 2000. Spectrophotometric assay of gene expression; Serratia marcescens pigmentation. Bioscene, 26(4):3-12
Lingappa K, Chandrashekhar Naik, Vivek Babu CS, Ramakrishna D, Venkata reddy M. 2002. A Novel substrate for citric acid production under solid state fermentation. Indian Journal of Microbiology 42:347-349.
Manderville RA. 2001. Synthesis, proton affinity and anticancer properties of the prodigiosin-group natural products. Curr. Medicinal Chem., 1(2):195-218.
Melvin PS, Elaine FM. 1973. Effect of iron and salt on prodigiosin synthesis in Serratia marcescens. J. Bacteriol., 114(3):99-1006
Min-Jung Song, Jungdon Bae, Dae Sil Lee, Chang-Ho Kim, Seung-Wook Kim, Suk-In Hong. 2006. Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95. J. Biosc. Bioeng., 101:157-161.
Montaner B, Navarro S, Pique M, Vilasca M, Martinell M, Giralt E, Gil J, Perez-Tomas R. 2000. Prodigiosin from the supernatant of Serratia marcescens induce apoptasis in haematopoietic cancer cell lines. Br. J. Pharmocol., 131:585-593.
Montaner B, Perez-Thomas R. 2001. Prodigiosin induced apoptosis in human colon cancer cells. Life Sci., 68:2025-2036.
Montserrat Sole, Nuria Rius, Alicia Francia, Loren JG. 1994. Letters in Applied Microbiology. 19:341-344.
Perez-Tomas R, Montaner B, Lalgostera E, Soto-cerrato V. 2003. The prodigiosins proapoptatic drugs with anti cancer properties. Biochem. Pharmocol., 66:1447-1452.
Pryce L Haddix, Terry F, Werner. 2000. Spectrophotometric assay of gene expression: Serratia marcescens Pigmentation. 26(4):3-13.
Rustom SM, Valiollah heidarynejad, Alka MP, Prafulla JD. 1990. Isolation and characterization of Serratia marcescens mutants defective in prodigiosin biosynthesis. Curr. Microbiol., 20:95-103.
Soto-Cerrato V, Lalgostera E, Montaner B, Scheffer GL, Perez-Thomas R. 2004. Mitochondria mediated apoptosis operating irrespective of multidrug resistance in breast cancer cells by the anticancer agent prodigiosin. Biochem. Phormocol., 68:1345-1352.
Sole M, Francia A, Rius N, Loren JG. 1997. The role of pH in the ‘glucose effect’ on prodigiosin production by non proliferating cells of Serratia marcescens. Lett. Appl. Microbio., 25:81-84.
Wei JR, Tsai YH, Horng YT, Soo PC, Hsieh SC, Ho SW, Lai HC. 2005. Biochemical characterization of RssA-RssB, a two component signal transuduction system regulating swarming behavior in Serratia marcescens. J. Bacteriol., 187:5683-5690.
Wei JR, Tsai YH, Horng YT, Soo PC, Hsieh SC, Hsueh PR, Horng JT, Williams P, Lai HC. 2006. A mobile quorum-sensing system in Serratia marcescens. J. Bacteriol., 188:1518-1525.
Yu-Hong Wei, Wei-Chuan Chen. 2005. Enhanced production of Prodigiosin like pigment from Serratia marcescens SMAR by medium improvement and oil-supplementation strategies. J. Biosc. Bio eng., 99(6):616-622.