Ursolic acid from leaf extracts of Barleria lupulina acting as anti clastogenic and anti tumor agent
Keywords:
Barleria lupulina, Acanthaceae family, anti-cancer, anti-tumor activities, ursolic acid, β sito sterol, sitosterol-3-O-glucoside
Abstract
In our previous study, we have reported the radio-protective, anti clastogenic and anti tumor activities of the leaf extract of Barleria lupulina on mice and fish. In the present work, biological active component from the leaf extract has been isolated, by column chromatography. Phytochemical analysis, TLC and NMR studies confirm the presence of ursolic acid as the main anti cancer component, besides other such components, as β sitosterol, sitosterol-3-O-glucoside are also present
References
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Carine J, Guillaume T, Alain D, Philippe T, Dirk P. 2006. In-vitro effects of polyphenols from cocoa and [beta]-sitosterol on the growth of human prostate cancer and normal cells. Eur J Cancer Prev., 15(4):353-361.
Furtado RA, Rodrigues ÉP, Araújo FR R, Oliveira WL, Furtado MA, Castro MB, Cunha WR, Tavares DC. 2008. Ursolic acid and oleanolic acid suppress preneoplastic lesions induced by 1,2-Dimethylhydrazine in rat colon. Tox Path. 36:576-580.
Gohar A, Gedara SR, Baraka HN. 2009. New acylated flavonol glycoside from Ceratonia siliqua L. seeds. J Med Plant Research. 3(5):424-448.
Jagetia GC, Venkatesha VA, Reddy TK. 2003. Naringin, a citrus flavonone, protects against radiation-induced chromosome damage in mouse bone marrow. Mutagenesis 18(4):337-343.
Jun L, Dong-mei W, Yuan-lin Z, Bin H, Zi-feng Z, Qin Y, Chan-min L, Qun S, Yong-jian W. 2010. Ursolic acid attenuates D-galactose-induced inflammatory response in mouse prefrontal cortex through inhibiting AGEs/RAGE/NF-kB pathway activation. Cer Cor., 20:2540-2548.
Kanchanapoom T, Kasai R, Yamasaki K. 2001. Iridoid glucosides from Barleria lupulina. Phytochemistry. 58:337-341.
Lai MY, Leung HWC, Yang WH, Chen WH, Lee HZ. 2007. Up-regulation of matrix metalloproteinase family gene involvement in ursolic acid-induced human lung non-small carcinoma cell apoptosis. Anticancer Res., 27:145-154.
Ovesná Z, Vachalkova A, Horvathova K, Tothova D. 2004. Pentacyclic triterpenoic acids: new chemoprotective compounds [minireview]. Neoplasma 51:327-33.
Pathak AK, Bhutani M, Nair AS, Ahn KS, Chakraborty A, Kadara H, Guha S, Sethi G, Aggarwal BB. 2007. Ursolic acid inhibits STAT3 activation pathway leading to suppression of proliferation and chemosensitization of Human Multiple Myeloma cells. Mol Cancer Res., 5:943-955.
Patt HM, Tyree EB, Straube RL, Smith DE. 1949. Cysteine protects against X-irradiation. Science. 110:213-214.
Suba V, Murugesan T, Rao RB, Ghosh L, Pal M, Mandal SC, Saha BP. 2004. Antidiabetic potential of Barleria lupulina extract in rats. Fitoterapia. 75(1):1-4.
Subbaramaiah K, Michaluart P, Sporn MB, Dannenberg AJ. 2000. Ursolic acid inhibits cyclooxygenase-2 transcription in human mammary epithelial cells. Cancer Res., 60:2399-2404.
Sur PK, Das PK. 2012. Radio-protective and anti-clastogenic effects of Barleria lupulina Lindl. extract against γ (gamma)-ray (1.2 Gy) induced mitotic chromosomal aberrations of laboratory mice Mus musculus and it’s effect on fish tumor induced after γ-irradiation. Journal of Research in Biology. 2(5):439-447.
Sur PK and Das PK. 2012. “A Process of Extracting Barleria lupulina Lindl. and the product thereof” (Patent). Patent No-639/KOL/2012, Date-07/06/2012, Govt. of India.
Trease GE and Evans WC. 1997. Pharmacognosy, fourteenth ed. Mac Millian Publ. Ltd., London.
Vasconcelos MA, Royo VA, Ferreira DS, Crotti AE, Silva MLA, Carvalho JC, Bastos JK, Cunha WR. 2006. In vivo analgesic and anti-inflammatory activities of ursolic acid and oleanolic acid from Miconia albicans. Z Naturforsch 61:477-82.
Mizushina Y, Nakanishi R, Kuriyama I, Kamiya K, Satake T. 2006. β-Sitosterol-3-O-β-d-glucopyranoside: A eukaryotic DNA polymerase λ inhibitor. J Steroid Biochem and Mol Bio., 99(2-3):100-107.
http://webprod.hc-sc.gc.ca/nhpid-bdipsn/ingredReq.do?id=3618&lang=eng
Carine J, Guillaume T, Alain D, Philippe T, Dirk P. 2006. In-vitro effects of polyphenols from cocoa and [beta]-sitosterol on the growth of human prostate cancer and normal cells. Eur J Cancer Prev., 15(4):353-361.
Furtado RA, Rodrigues ÉP, Araújo FR R, Oliveira WL, Furtado MA, Castro MB, Cunha WR, Tavares DC. 2008. Ursolic acid and oleanolic acid suppress preneoplastic lesions induced by 1,2-Dimethylhydrazine in rat colon. Tox Path. 36:576-580.
Gohar A, Gedara SR, Baraka HN. 2009. New acylated flavonol glycoside from Ceratonia siliqua L. seeds. J Med Plant Research. 3(5):424-448.
Jagetia GC, Venkatesha VA, Reddy TK. 2003. Naringin, a citrus flavonone, protects against radiation-induced chromosome damage in mouse bone marrow. Mutagenesis 18(4):337-343.
Jun L, Dong-mei W, Yuan-lin Z, Bin H, Zi-feng Z, Qin Y, Chan-min L, Qun S, Yong-jian W. 2010. Ursolic acid attenuates D-galactose-induced inflammatory response in mouse prefrontal cortex through inhibiting AGEs/RAGE/NF-kB pathway activation. Cer Cor., 20:2540-2548.
Kanchanapoom T, Kasai R, Yamasaki K. 2001. Iridoid glucosides from Barleria lupulina. Phytochemistry. 58:337-341.
Lai MY, Leung HWC, Yang WH, Chen WH, Lee HZ. 2007. Up-regulation of matrix metalloproteinase family gene involvement in ursolic acid-induced human lung non-small carcinoma cell apoptosis. Anticancer Res., 27:145-154.
Ovesná Z, Vachalkova A, Horvathova K, Tothova D. 2004. Pentacyclic triterpenoic acids: new chemoprotective compounds [minireview]. Neoplasma 51:327-33.
Pathak AK, Bhutani M, Nair AS, Ahn KS, Chakraborty A, Kadara H, Guha S, Sethi G, Aggarwal BB. 2007. Ursolic acid inhibits STAT3 activation pathway leading to suppression of proliferation and chemosensitization of Human Multiple Myeloma cells. Mol Cancer Res., 5:943-955.
Patt HM, Tyree EB, Straube RL, Smith DE. 1949. Cysteine protects against X-irradiation. Science. 110:213-214.
Suba V, Murugesan T, Rao RB, Ghosh L, Pal M, Mandal SC, Saha BP. 2004. Antidiabetic potential of Barleria lupulina extract in rats. Fitoterapia. 75(1):1-4.
Subbaramaiah K, Michaluart P, Sporn MB, Dannenberg AJ. 2000. Ursolic acid inhibits cyclooxygenase-2 transcription in human mammary epithelial cells. Cancer Res., 60:2399-2404.
Sur PK, Das PK. 2012. Radio-protective and anti-clastogenic effects of Barleria lupulina Lindl. extract against γ (gamma)-ray (1.2 Gy) induced mitotic chromosomal aberrations of laboratory mice Mus musculus and it’s effect on fish tumor induced after γ-irradiation. Journal of Research in Biology. 2(5):439-447.
Sur PK and Das PK. 2012. “A Process of Extracting Barleria lupulina Lindl. and the product thereof” (Patent). Patent No-639/KOL/2012, Date-07/06/2012, Govt. of India.
Trease GE and Evans WC. 1997. Pharmacognosy, fourteenth ed. Mac Millian Publ. Ltd., London.
Vasconcelos MA, Royo VA, Ferreira DS, Crotti AE, Silva MLA, Carvalho JC, Bastos JK, Cunha WR. 2006. In vivo analgesic and anti-inflammatory activities of ursolic acid and oleanolic acid from Miconia albicans. Z Naturforsch 61:477-82.
Mizushina Y, Nakanishi R, Kuriyama I, Kamiya K, Satake T. 2006. β-Sitosterol-3-O-β-d-glucopyranoside: A eukaryotic DNA polymerase λ inhibitor. J Steroid Biochem and Mol Bio., 99(2-3):100-107.
http://webprod.hc-sc.gc.ca/nhpid-bdipsn/ingredReq.do?id=3618&lang=eng
Published
2012-10-29
How to Cite
Das, P. K., & Sur, P. K. (2012). Ursolic acid from leaf extracts of Barleria lupulina acting as anti clastogenic and anti tumor agent. Journal of Research in Biology, 2(7), 667-675. Retrieved from https://ojs.jresearchbiology.com/ojs1/index.php/jrb/article/view/265
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Articles
